Population encoding by circadian clock neurons organizes circadian behavior.
Population encoding by circadian clock neurons organizes circadian behavior.
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DOI：
10.1523/jneurosci.3801-08.2009
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发表时间：
2009-02-11
期刊：
影响因子：
--
通讯作者：
McMahon DG
中科院分区：
文献类型：
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作者：
Ciarleglio CM;Gamble KL;Axley JC;Strauss BR;Cohen JY;Colwell CS;McMahon DG
哺乳动物的昼夜节律是由下丘脑的视交叉上核（SCN）协调的。SCN由生物钟神经元组成，但这些神经元振荡器编码节律行为的机制尚不完全清楚。我们已经使用离体实时基因表达成像的昼夜节律行为的神经相关性，结合血管活性肠多肽（VIP），一个关键的SCN信号分子的遗传破坏，检查在SCN的昼夜节律组织的神经基础。我们发现，时钟神经元节律的连贯性和定时与个体小鼠内行为节律的连贯性和定时相关，SCN神经元组织的破坏程度与个体内行为破坏的程度相关。我们的研究结果表明，SCN编码的昼夜节律相位作为其组成神经元的时间人口矢量，这样的神经元人口变得deserminized，相位信息变得模糊。
Mammalian circadian rhythms are orchestrated by the suprachiasmatic nuclei (SCN) of the hypothalamus. The SCN are composed of circadian clock neurons but the mechanisms by which these populations of neuronal oscillators encode rhythmic behavior are incompletely understood. We have used ex vivo real-time gene expression imaging of the neural correlates of circadian behavior, combined with genetic disruption of vasoactive intestinal polypeptide (VIP), a key SCN signaling molecule, to examine the neural basis of circadian organization in the SCN. We show that the coherence and timing of clock neuron rhythms are correlated with the coherence and timing of behavioral rhythms within individual mice, and that the degree of disruption of SCN neuronal organization correlates with the degree of behavioral disruption within individuals. Our results suggest that the SCN encode circadian phase as a temporal population vector of its constituent neurons such that as the neuronal population becomes desynchronized, phase information becomes ambiguous.