Effects of treadmill exercise-intensity on short-term memory in the rats born of the lipopolysaccharide-exposed maternal rats.
Effects of treadmill exercise-intensity on short-term memory in the rats born of the lipopolysaccharide-exposed maternal rats.
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DOI：
10.12965/jer.150264
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发表时间：
2015-12
影响因子：
1.9
通讯作者：
Chung YR
中科院分区：
文献类型：
--
作者：
Kim K;Sung YH;Seo JH;Lee SW;Lim BV;Lee CY;Chung YR
母体感染是导致新生儿脑损伤和后期发育障碍的重要因素。在本研究中，我们研究了跑台运动强度对大鼠短期记忆，海马神经发生，脑源性神经营养因子（BDNF）和酪氨酸激酶受体B（Trk B）的表达的影响。将大鼠分为6组：对照组、中等强度运动组、中等强度运动组、母体内毒素暴露组、母体内毒素暴露+中等强度运动组、母体内毒素暴露+中等强度运动组。运动组大鼠每周5次，每次30 min，连续4周进行跑台运动。运动负荷包括轻度运动组以8 m/min的速度跑步，中度运动组以14 m/min的速度跑步。采用跳台回避法测定短时记忆的潜伏期。免疫组化5-溴-钼-2 ′-脱氧尿苷检测海马细胞增殖和神经发生。Western blot检测BDNF和TrkB的表达。在本研究中，跑步机运动改善了母亲LPS暴露导致的短期记忆恶化。跑台运动增加了LPS暴露母鼠所生大鼠海马齿状回的细胞增殖和神经发生。跑台运动增加了LPS暴露母鼠所生大鼠海马中BDNF和TrkB的表达。跑台运动的这些效果在中等强度和中等强度下同样出现。
Maternal infection is an important factor causing neonatal brain injury and later developmental disability. In the present study, we investigated the effects of treadmill exercise intensity on short-term memory, hippocampal neurogenesis, and expression of brain-derived neurotrophic factor (BDNF), and tyrosine kinase receptor B (TrkB) in the rats born of lipopolysaccharide (LPS)-exposed maternal rats. The rats were divided into six groups: control group, mild-intensity exercise group, moderate-intensity exercise group, maternal LPS-exposed group, maternal LPS-exposed and mild-intensity exercise group, maternal LPS-exposed and moderate-intensity exercise group. The rats in the exercise groups were forced to run on a treadmill for 30 min 5 times a week for 4 weeks. The exercise load consisted of running at the speed of 8 m/min for the mild-intensity exercise groups and 14 m/min for moderate-intensity exercise groups. The latency in the step-down avoidance task was deter-mined for the short-term memory. Immunohistochemistry for 5-bro-mo-2′-deoxyuridine was performed to determine hippocampal cell proliferation and neurogenesis. Western blot analysis was performed for the detection of BDNF and TrkB expression. In the present study, tread-mill exercise improved short-term memory deteriorated by maternal LPS exposure. Treadmill exercise increased cell proliferation and neurogenesis in the hippocampal dentate gyrus of the rats born of the LPS-exposed maternal rats. Treadmill exercise increased BDNF and TrkB expression in the hippocampus of the rats born of the LPS-exposed maternal rats. These effects of treadmill exercise were similarly appeared at both mild-intensity and moderate-intensity.