High-resolution analysis of chromosome arm 1p alterations in meningioma
High-resolution analysis of chromosome arm 1p alterations in meningioma
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DOI：
10.1016/s0165-4608(99)00249-6
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发表时间：
2000-07-01
影响因子：
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通讯作者：
Rey, JA
中科院分区：
文献类型：
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作者：
Bello, MJ;de Campos, JM;Rey, JA
在人类脑膜瘤中，染色体臂1p基因的杂合性缺失(LOH)是一种比较常见的事件，这种异常被认为与II级或III级脑膜瘤(不典型和间变性脑膜瘤)的发生有关。然而，现有数据有限，无法确定受影响的1P区域的频率和范围。为了确定1p染色体在脑膜瘤中的地位，我们利用高密度的Ip标记基因座对100例脑膜瘤进行了Ip上的LOH综合分析。在35%的肿瘤中发现了等位基因丢失，大多数对应于不典型的脑膜瘤，这些脑膜瘤也显示了22号染色体上的基因缺失。虽然有些肿瘤表现出复杂的重排，导致不同的Ip缺失，但丢失的模式显示了两个主要靶区：1p36和1p34-p32，这是最常见的受累区域，而在一些肿瘤中，1p22和1p21.1-1p13区域出现缺失。这些结果表明，Ip上可能存在几个可能的肿瘤抑制基因，这些基因的失活可能在脑膜瘤的发病机制中以及在其他类型的肿瘤中起重要作用。(C)2000 Elsevier Science Inc.保留所有权利。
Loss of heterozygosity (LOH) for loci on chromosome arm 1p is a relatively common event in human meningioma, and this anomaly has been proposed to be associated with the development of grade II or grade III forms (atypical and anaplastic meningiomas). Nevertheless the limited data available do not allow the establishment of the frequency and the extent of the affected 1p regions. To determine the status of chromosome 1p in meningiomas, we have per;formed a comprehensive analysis of LOH on Ip in 100 meningiomas using a high density of Ip-marker loci. Allelic loss was found in 35% of tumors, most corresponding to nontypical meningiomas that also displayed losses for loci on chromosome 22. Although some tumors displayed complex rearrangements leading to distinct Ip deletions, the patterns of loss indicated two main target regions: 1p36 and 1p34-p32, which represent the most frequently involved regions, whereas 1p22 and 1p21.1-1p13 regions appeared deleted in some tumors. These results suggest that there may be several putative tumor suppressor genes on Ip, the inactivation of which may be important in the pathogenesis of meningiomas, as well as in other tumor types. (C) 2000 Elsevier Science Inc. All rights reserved.