In vitro methylation of HpaII sites in Xenopus laevis rDNA does not affect its transcription in oocytes.
In vitro methylation of HpaII sites in Xenopus laevis rDNA does not affect its transcription in oocytes.
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非洲爪蟾 rDNA 中 HpaII 位点的体外甲基化不影响其在卵母细胞中的转录。
DOI：
10.1093/nar/12.4.2225
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发表时间：
1984
影响因子：
14.9
通讯作者：
Reeder,RH
中科院分区：
文献类型：
--
作者：
Pennock,DG;Reeder,RH
通过甲基化一系列rDNA质粒中的所有Bpa Ⅱ位点，并将甲基化的rDNA注射到X. borealisocytes中，检测了X.laevisrDNA甲基化对其转录的影响。从注射的质粒中转录的RNA分析表明，rDNA的甲基化不影响非洲爪蟾核糖体基因的转录。这一结果表明，尽管观察到了黄鳍木中rDNA的去甲基化与核糖体RNA合成之间的相关性（1），但rDNA的甲基化并不调节基因的转录。
The effect of methylation ofX.laevisrDNA on its transcription was tested by methylating all of theBpaII sites in a series of rDNA plasmids and injecting the methylated rDNA intoX.borealisoocytes. Analysis of RNA transcribed from the injected plasmids showed that methylation of rDNAinvitrodoes not affect transcription ofX.laevisribosomal genes. This result suggests that despite the observed correlation between demethylation of rDNA and synthesis of ribosomal RNA inX.laevisembryos (1), methylation of rDNA does not regulate transcription of the gene.