A WD40-repeat gene from Malus x domestica is a functional homologue of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1
A WD40-repeat gene from Malus x domestica is a functional homologue of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1
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DOI：
10.1007/s00299-010-0821-0
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发表时间：
2010-03-01
影响因子：
6.2
通讯作者：
Sagasser, Martin
中科院分区：
文献类型：
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作者：
Brueggemann, Julian;Weisshaar, Bernd;Sagasser, Martin
WD 40重复蛋白透明种皮GLABRA1（TTG1）参与拟南芥的许多发育和生化反应，例如种皮颜色和粘液的产生、花青素的着色以及毛状体和根毛的形成。在这项研究中，一个假定的TTG 1同源物分离自苹果（Malus x arctica Borkh.）与A. thaliana TTG 1的核苷酸序列和氨基酸序列的同源性为90.7%。MdTTG1候选基因能够与A.拟南芥转录因子TT2和TT8的表达。拟南芥原生质体。这表明编码的蛋白质可以整合到激活A中BAN的复合物中。thaliana，苹果中也可能存在类似的复合物。转化到A.在拟南芥中，苹果序列能够恢复毛状体生长、幼苗中的花色素苷产生以及种子中的原花色素产生。此外，补充突变体植物的根显示类似于野生型的根毛形成。这些结果表明，所研究的苹果WD40基因是AtTTG1的功能同源物，我们将该基因称为MdTTG1。
The WD40 repeat protein TRANSPARENT TESTA GLABRA1 (TTG1) is involved in a multitude of developmental and biochemical reactions in Arabidopsis thaliana such as the production of seed coat colour and mucilage, pigmentation by anthocyanins as well as the formation of trichomes and root hairs. In this study, a putative TTG1 homologue was isolated from apple (Malus x domestica Borkh.) showing 80.2% identity to A. thaliana TTG1 on nucleotide and 90.7% similarity on amino acid level. The MdTTG1 candidate was able to activate the AtBAN promoter in cooperation with the A. thaliana transcription factors TT2 and TT8 in A. thaliana protoplasts. This indicates that the encoded protein can be integrated into the complex that activates BAN in A. thaliana, and that a similar complex might also be present in apple. When transformed into ttg1 mutants of A. thaliana, the apple sequence was able to restore trichome growth, anthocyanin production in young seedlings as well as proanthocyanidin production in seeds. Additionally, roots of complemented mutant plants showed root hair formation resembling wild type. These results show that the studied apple WD40 gene is a functional homologue of AtTTG1 and we refer to this gene as MdTTG1.