Fluorescence lifetime imaging ophthalmoscopy.
Fluorescence lifetime imaging ophthalmoscopy.
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DOI：
10.1016/j.preteyeres.2017.06.005
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发表时间：
2017-09
影响因子：
17.8
通讯作者：
Zinkernagel MS
中科院分区：
文献类型：
--
作者：
Dysli C;Wolf S;Berezin MY;Sauer L;Hammer M;Zinkernagel MS
基于视网膜自身荧光的成像技术由于其非常敏感和无创性而在眼科中得到了广泛的应用。常规眼底自身荧光成像测量内源性视网膜荧光团的荧光强度。其信号主要来源于视网膜色素上皮水平的脂褐素。而眼底自身荧光不仅可以通过荧光强度或发射光谱的空间分布来表征，还可以通过特征荧光寿命函数来表征。荧光寿命是荧光团在激发后保持在激发态的平均时间。荧光寿命成像眼科检查（FLIO）是一种新兴的成像方式，用于体内测量内源性视网膜荧光团的寿命。最近在这一领域的报道有助于我们对各种黄斑和视网膜疾病的病理生理学的理解。本文介绍了荧光寿命成像的基本概念。它包括技术背景信息和与个体视网膜代谢物的体外测量的相关性。在第二部分，荧光寿命成像的临床应用和荧光寿命特征的选择视网膜疾病，如Stargardt病，年龄相关性黄斑变性，脉络膜血症，中央浆液性脉络膜视网膜病变，黄斑孔，糖尿病视网膜病变，视网膜动脉闭塞。荧光寿命成像检眼镜的潜在应用领域将在本综述的最后概述。
Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can not only be characterized by the spatial distribution of the fluorescence intensity or emission spectrum, but also by a characteristic fluorescence lifetime function. The fluorescence lifetime is the average amount of time a fluorophore remains in the excited state following excitation. Fluorescence lifetime imaging ophthalmoscopy (FLIO) is an emerging imaging modality for in vivo measurement of lifetimes of endogenous retinal fluorophores. Recent reports in this field have contributed to our understanding of the pathophysiology of various macular and retinal diseases. Within this review, the basic concept of fluorescence lifetime imaging is provided. It includes technical background information and correlation with in vitro measurements of individual retinal metabolites. In a second part, clinical applications of fluorescence lifetime imaging and fluorescence lifetime features of selected retinal diseases such as Stargardt disease, age-related macular degeneration, choroideremia, central serous chorioretinopathy, macular holes, diabetic retinopathy, and retinal artery occlusion are discussed. Potential areas of use for fluorescence lifetime imaging ophthalmoscopy will be outlined at the end of this review.
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