Molecular cloning and sequences of cDNAs encoding α (large) and β (small) isoforms of human pancreatic zymogen granule membrane-associated protein GP2
Molecular cloning and sequences of cDNAs encoding α (large) and β (small) isoforms of human pancreatic zymogen granule membrane-associated protein GP2
复制标题
DOI：
10.1016/s0167-4781(00)00057-9
复制
发表时间：
2000-04-25
期刊：
影响因子：
--
通讯作者：
Fukuoka, SI
中科院分区：
文献类型：
--
作者：
Fukuoka, SI
已分离出编码两种不同（α 和 β）形式的人胰腺酶原颗粒膜相关蛋白糖蛋白 2 (GP2)（也称为 ZAP75）的 cDNA 克隆，GP2 是胰腺腺泡细胞中沿顶端分泌途径调节膜运输的关键成分。克隆的结构分析表明，GP2 的 α 和 β 形式分别由 527 个和 380 个氨基酸 (aa) 残基组成。 beta 形式缺少与 a 形式的 25-171 区域相对应的 147 个氨基酸结构域，表明它是选择性剪接事件的产物。证实了两种形式的 GP2 在人胰腺中的表达。 GP2 的独特亚型。是首次在人类中报道。 (C) 2000 Elsevier Science B.V. 保留所有权利。
cDNA clones encoding two different (alpha and beta) forms of human pancreatic zymogen granule membrane-associated protein glycoprotein 2 (GP2) (also referred to as ZAP75), a critical component in regulated membrane trafficking along the apical secretory pathway in pancreatic acinar cells, have been isolated. Structural analysis of the clones revealed that the alpha and beta forms of GP2 consist of 527 and 380 amino acid (aa) residues, respectively. The beta form lacks a 147 aa domain that corresponds to the 25-171 region of the a form, suggesting that it is a product of an alternative splicing event. Expression of both forms of GP2 in the human pancreas was confirmed. A unique isoform of GP2. is reported for the first time in humans. (C) 2000 Elsevier Science B.V. All rights reserved.