Development of a serodiagnostic test for sheep scab using recombinant protein Pso o 2
Development of a serodiagnostic test for sheep scab using recombinant protein Pso o 2
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DOI：
10.1016/j.mcp.2011.09.002
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发表时间：
2011-10-01
影响因子：
3.3
通讯作者：
Huntley, John F.
中科院分区：
文献类型：
--
作者：
Nunn, Francesca G.;Burgess, Stewart T. G.;Huntley, John F.
早期阶段的羊疥疮，疾病引起的非穴居螨羊痒螨，往往是亚临床，或可能被误诊。因此，在疾病控制中非常需要能够检测早期疾病和潜伏侵染的诊断测试。本文介绍了一种用重组羊疟原虫抗原（Pso o 2）建立的早期检测绵羊抗羊疟原虫血清抗体的ELISA方法的设计和验证。使用来自感染绵羊疟原虫的绵羊（n = 58）和没有感染绵羊疟原虫的绵羊（n = 433）以及感染其他寄生虫（包括胃肠道线虫（GIN）或咀嚼虱）的绵羊的血清评价该ELISA。使用491份绵羊血清的ELISA结果生成受试者工作特征（ROC）曲线分析，曲线下面积（AUC）为0.97。>0.06吸光度单位的最佳OD（450）截止值给出0.93的测试灵敏度和0.90的特异性。基于P50_02的ELISA能够在疾病开放之前的早期实验感染期间检测绵羊疟原虫的特异性抗体，表明其用于检测亚临床感染的实用性。Crown版权所有（C）2011由Elsevier Ltd.发布。保留所有权利。
Early stages of sheep scab, the disease caused by the non-burrowing mite Psoroptes ovis, are often sub-clinical, or can be mis-diagnosed. A diagnostic test capable of detecting early disease and latent infestations is therefore highly desirable in disease control. This paper describes the design and validation of an ELISA, which incorporates a recombinant P. ovis antigen (Pso o 2), for the early detection of anti-P. ovis serum antibodies in sheep. This ELISA was evaluated using sera from sheep infested with P ovis (n = 58) and sheep (n = 433) with no P. ovis infestation as well as sheep infected with other parasites including gastrointestinal nematodes (GIN), or chewing lice. A receiver operating characteristic (ROC) curve analysis was generated using the ELISA results for 491 sheep sera with the area under the curve (AUC) being 0.97. An optimal OD(450) cut-off of >0.06 absorbance units gave a test sensitivity of 0.93 and specificity of 0.90. The Pso o 2-based ELISA was able to detect specific antibodies to P. ovis during early experimental infestation prior to disease patency, indicating its utility for detecting sub-clinical infestation. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.