Comparative study on the effects of cigarette smoke exposure, ethanol consumption and association: Behavioral parameters, apoptosis, glial fibrillary acid protein and S100β immunoreactivity in different regions of the rat hippocampus
Comparative study on the effects of cigarette smoke exposure, ethanol consumption and association: Behavioral parameters, apoptosis, glial fibrillary acid protein and S100β immunoreactivity in different regions of the rat hippocampus
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DOI：
10.1016/j.alcohol.2018.08.009
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发表时间：
2019-06-01
期刊：
影响因子：
2.3
通讯作者：
Fernandes, Marilda da Cruz
中科院分区：
文献类型：
--
作者：
Huf, Fernanda;Bandiera, Solange;Fernandes, Marilda da Cruz
暴露在香烟烟雾和酒精中被认为会引发神经毒性、细胞凋亡，并损害神经元信号转导。然而，目前尚不清楚两者的结合如何可能引发星形胶质细胞增生症，以及能够影响海马区对这些合法药物的不同敏感性的形态变化。本研究观察了香烟烟雾和/或酒精暴露对大鼠海马齿状回(DG)、CA1区和CA3区行为参数、细胞凋亡以及胶质纤维酸性蛋白(GFAP)和S100β免疫反应活性的影响。成年雄性Wistar大鼠32只，随机分为4组：VE组、TDB组、乙醇+乙醇组和TOB+乙醇+酒精组，每组54 d。这些组被要求进行甩尾、开阔场地和抑制性回避任务。结果表明，酒精本身会恶化短期记忆。TOB和乙醇的联合作用增加了CA3和DG区裂解的caspase-3的免疫反应性。TOB+乙醇组大鼠海马区GFAP免疫反应均较弱。此外，乙醇和TOB本身也降低了DG内GFAP的免疫反应。乙醇只增加DG的S100β免疫反应性。总而言之，这项研究表明，只有酒精会恶化短期记忆，DG变得更容易受到所研究标记物变化的影响。这一证据表明，DG对香烟烟雾和乙醇引起的神经毒性更敏感。(C)2018 Elsevier Inc.保留所有权利。
Exposure to cigarette smoke and ethanol are proposed to trigger neurotoxicity, apoptosis, and to impair neuronal signaling. However, it is little known how the combination of both might trigger astrogliosis and the morphological changes capable of affecting a differential susceptibility of hippocampal regions to these licit drugs. The present study investigated the chronic effects of exposure to cigarette smoke and/or ethanol on behavioral parameters, apoptosis, and alteration in immunoreactivity of glial fibrillary acid protein (GFAP) and S100 beta in the CA1, CA3, and dentate gyrus (DG) of the rat hippocampus. Adult male Wistar rats (n = 32) were divided into four groups: vehicle (VE, glucose 3% in water, 10 mL/kg), cigarette smoke (TDB, total 12 cigarettes per day), ethanol (ethanol, 2 g/kg), and cigarette smoke plus ethanol (TOB plus ethanol, total 12 cigarettes per day plus ethanol 2 g/kg) for 54 days. The groups were submitted to tail-flick, open-field, and inhibitory avoidance tasks. The results showed that ethanol per se worsened the short-term memory. The association between TOB and ethanol increased the immunoreactivity of cleaved caspase-3 in the CA3 and DG regions. The TOB plus ethanol group showed a lower immunoreactivity to GFAP in all regions of the hippocampus. In addition, ethanol and TOB per se also reduced the immunoreactivity for GFAP in the DG. Ethanol increased S100 beta immunoreactivity only in the DG. In conclusion, this study showed that only ethanol worsened short-term memory, and the DG became more susceptible to changes in the markers investigated. This evidence suggests that DG is more sensitive to neurotoxicity induced by cigarette smoke and ethanol. (C) 2018 Elsevier Inc. All rights reserved.