Biological effect of hydrothermally synthesized silica nanoparticles within crystalline hydroxyapatite coatings for titanium implants
Biological effect of hydrothermally synthesized silica nanoparticles within crystalline hydroxyapatite coatings for titanium implants
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DOI：
10.1016/j.msec.2018.06.043
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发表时间：
2018-11-01
影响因子：
7.9
通讯作者：
Marszalek, Marta
中科院分区：
文献类型：
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作者：
Bartkowiak, Amanda;Suchanek, Katarzyna;Marszalek, Marta
生物医学领域迫切需要开发用于人工骨植入物的功能性涂层，以增强骨结合并加速骨愈合过程。在这项研究中，我们提出了新的复合涂层的生物学效应与不同浓度的二氧化硅纳米粒子内结晶羟基磷灰石基质（HAp-SiO2）在水热条件下合成的钛。分析样品的元素组成、结构、生物活性和体外细胞毒性。结果表明，纳米二氧化硅颗粒在六方羟基磷灰石（HAp）晶体表面形成并均匀分布。在模拟体液（SBF）中浸泡4天后，与纯HAp相比，涂层显示出改善的生物活性。人成骨样细胞（MG-63）在合成材料上培养的反应提供了证据，HAp-SiO2复合材料具有良好的生物相容性。我们认为这是因为HAp-SiO2复合材料有利于生物矿化过程，细胞增殖保持不受影响，无论二氧化硅的量如何。此外，SEM和荧光测量表明，HAp-SiO2对细胞形态有积极的影响，有利于细胞粘附。
Development of functional coatings for artificial bone implants that strengthen the osseointegration and accelerate bone healing processes is urgently needed in the biomedical field. In this study we present biological effect of novel composite coatings with different concentration of silica nanoparticles within crystalline hydroxyapatite matrix (HAp-SiO2 ) synthesized on titanium under hydrothermal conditions. Samples were analyzed for their elemental composition, structure, bioactivity and in vitro cytotoxicity. The results indicate the formation and homogeneous distribution of silica nanoparticles on the surface of hexagonal hydroxyapatite (HAp) crystals. The coatings show improved bioactivity in comparison with pure HAp after 4 days of immersion in simulated body fluid (SBF). The responses of human osteoblast-like cells (MG-63) cultured onto the synthesized materials provide evidence that HAp-SiO2 composites exhibit good biocompatibility. We propose that this is because HAp-SiO2 composites favor biomineralization process with cell proliferation remaining unaffected, regardless of the amount of silica. Furthermore, SEM and fluorescence measurements demonstrate that HAp-SiO2 had positive effect on cell morphology, favoring cell adhesion.