Substrate binding protein SBP2 of a putative ABC transporter as a novel vaccine antigen of Moraxella catarrhalis.
Substrate binding protein SBP2 of a putative ABC transporter as a novel vaccine antigen of Moraxella catarrhalis.
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假定的 ABC 转运蛋白的底物结合蛋白 SBP2 作为卡他莫拉氏菌的新型疫苗抗原。
DOI：
10.1128/iai.01832-14
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发表时间：
2014
影响因子：
3.1
通讯作者：
Murphy,TimothyF
中科院分区：
文献类型：
--
作者：
Otsuka,Taketo;Kirkham,Charmaine;Johnson,Antoinette;Jones,MeganM;Murphy,TimothyF
卡他莫拉菌是一种常见的呼吸道病原体，可导致儿童中耳炎和成人慢性阻塞性肺疾病感染。自从非分型流感嗜血杆菌D蛋白结合疫苗问世以来，卡他分支杆菌已成为中耳炎的首选致病菌。对于抗菌疫苗和治疗的开发，ATP结合盒转运蛋白的底物结合蛋白是重要的靶点。在这项研究中，我们鉴定并鉴定了卡他分支杆菌的底物结合蛋白SBP2。在30株临床分离株中，bp2基因序列高度保守。在两种不同的分析(全细胞酶联免疫吸附试验和流式细胞术)中，针对重组SBP2的多克隆抗体表明，SBP2表达野生型细菌表面的表位，而不表达bp2突变体的表位。在25-μg和50-μg剂量下，重组SBP2免疫的小鼠与对照组相比，卡他分支杆菌从肺中的清除量显著增加(P<；0.001)。我们的结论是，SBP2是一种新的、有吸引力的卡氏支原体疫苗抗原。
Moraxella catarrhalis is a common respiratory tract pathogen that causes otitis media in children and infections in adults with chronic obstructive pulmonary disease. Since the introduction of the pneumococcal conjugate vaccines with/without protein D of nontypeable Haemophilus influenzae, M. catarrhalis has become a high-priority pathogen in otitis media. For the development of antibacterial vaccines and therapies, substrate binding proteins of ATP-binding cassette transporters are important targets. In this study, we identified and characterized a substrate binding protein, SBP2, of M. catarrhalis. Among 30 clinical isolates tested, thesbp2gene sequence was highly conserved. In 2 different analyses (whole-cell enzyme-linked immunosorbent assay and flow cytometry), polyclonal antibodies raised to recombinant SBP2 demonstrated that SBP2 expresses epitopes on the bacterial surface of the wild type but not thesbp2mutant. Mice immunized with recombinant SBP2 showed significantly enhanced clearance of M. catarrhalis from the lung compared to that in the control group at both 25-μg and 50-μg doses (P< 0.001). We conclude that SBP2 is a novel, attractive candidate as a vaccine antigen against M. catarrhalis.