Effect of intestinal microflora and dietary fat on 3,2'-dimethyl-4-aminobiphenyl-induced colon carcinogenesis in F344 rats.
Effect of intestinal microflora and dietary fat on 3,2'-dimethyl-4-aminobiphenyl-induced colon carcinogenesis in F344 rats.
复制标题
肠道微生物群和膳食脂肪对 3,2-二甲基-4-氨基联苯诱导的 F344 大鼠结肠癌的影响。
作者：
B. Reddy;T. Ohmori
以断奶雄性F344无菌大鼠和普通大鼠分别饲喂低脂和高脂半纯化日粮，研究了饲粮脂肪和肠道菌群对结肠和小肠癌变的影响。7周龄时，除载药组外，所有动物均按每kg体重每周注射50 mg 3,2'-二甲基-4-氨基联苯（DMAB），持续20周。在最后一次注射DMAB 20周后，用DMAB和载体处理的无菌大鼠和喂食高脂和低脂饮食的常规大鼠进行尸检。无菌大鼠结肠、小肠、耳道和皮肤肿瘤的发生率低于常规动物。饲喂高脂饲料的常规大鼠dmab诱导的结肠肿瘤的发生率和多样性高于饲喂低脂饲料的常规大鼠；然而，高脂和低脂喂养的无菌动物的肿瘤发病率没有显著差异。饮食脂肪对无菌大鼠和常规大鼠小肠肿瘤的发生率均无影响。
The effect of dietary fat and intestinal microflora on colon and small intestinal carcinogenesis was studied in weanling male F344 germfree and conventional rats fed semipurified diets containing low and high fat. At 7 weeks of age, all animals, except vehicle-treated groups, received weekly s.c. injections of 50 mg 3,2'-dimethyl-4-aminobiphenyl (DMAB) per kg body weight for 20 weeks. The DMAB- and vehicle-treated germfree and conventional rats fed high- and low-fat diets were autopsied 20 weeks after the last injection of DMAB. The incidence of colon, small intestinal, ear duct, and skin tumors was lower in germfree rats than in the conventional animals. The incidence and multiplicity of DMAB-induced colon tumors were higher in conventional rats fed the high-fat diet than in the conventional animals fed the low-fat diet; however, the tumor incidence did not differ significantly between the high- and low-fat-fed germfree animals. Dietary fat had no effect on the incidence of small intestinal tumors in both germfree and conventional rats.