Development of an X-ray fluorescence holographic measurement system for protein crystals.
Development of an X-ray fluorescence holographic measurement system for protein crystals.
复制标题
开发蛋白质晶体的 X 射线荧光全息测量系统。
DOI：
10.1063/1.4953453
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
K. Hayashi
中科院分区：
文献类型：
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作者：
A. Sato‐Tomita;N. Shibayama;N. Happo;K. Kimura;Takahiro Okabe;T. Matsushita;Sam;Y. Sasaki;K. Hayashi
描述了获得晶体金属蛋白样品X射线荧光全息图的实验步骤和装置。人类血红蛋白是一种α2β2四聚体金属蛋白，每条链中含有Fe（II）血红素活性位点，由于其丰富的晶体学数据而被选择用于本研究。引入冷气流系统以减少通常易碎且易受损伤的蛋白质晶体的X射线辐射损伤。安装X-台以旋转样品，同时避免X射线束与样品环或保持器之间的交叉，这是支撑易碎蛋白质晶体所需的。制备了巨大的血红蛋白晶体（最大尺寸为8 × 6 × 3 mm（3）），并用于在整个测量过程中保持入射X射线束的足迹小于样品尺寸，入射角为0°-70°。在这些实验和数据采集条件下，我们实现了从蛋白质晶体的X射线荧光全息图图案的首次观察，具有最小的辐射损伤，开辟了一个新的和潜在的方法，研究生物大分子中的金属活性位点的立体化学。
Experimental procedure and setup for obtaining X-ray fluorescence hologram of crystalline metalloprotein samples are described. Human hemoglobin, an α2β2 tetrameric metalloprotein containing the Fe(II) heme active-site in each chain, was chosen for this study because of its wealth of crystallographic data. A cold gas flow system was introduced to reduce X-ray radiation damage of protein crystals that are usually fragile and susceptible to damage. A χ-stage was installed to rotate the sample while avoiding intersection between the X-ray beam and the sample loop or holder, which is needed for supporting fragile protein crystals. Huge hemoglobin crystals (with a maximum size of 8 × 6 × 3 mm(3)) were prepared and used to keep the footprint of the incident X-ray beam smaller than the sample size during the entire course of the measurement with the incident angle of 0°-70°. Under these experimental and data acquisition conditions, we achieved the first observation of the X-ray fluorescence hologram pattern from the protein crystals with minimal radiation damage, opening up a new and potential method for investigating the stereochemistry of the metal active-sites in biomacromolecules.
利用 X 射线荧光全息技术分析千层状 Mg-Zn-Y 合金的局部结构
DOI：
--
发表时间：
2019
期刊：
影响因子：
--
作者：
K. Kimura;T. Nishioka;Y. Yamamoto;K. Hagihara;H. Izumo;N. Happo;S. Hosokawa;E. Abe;M. Suzuki;T. Matsushita;and K. Hayashi
通讯作者：
and K. Hayashi