Differential roles of the fan-shaped body and the ellipsoid body in Drosophila visual pattern memory
Differential roles of the fan-shaped body and the ellipsoid body in Drosophila visual pattern memory
复制标题
扇形体和椭球体在果蝇视觉模式记忆中的不同作用
DOI：
10.1101/lm.1331809
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发表时间：
2009-05-01
影响因子：
2
通讯作者：
Liu, Li
中科院分区：
文献类型：
--
作者：
Pan, Yufeng;Zhou, Yanqiong;Liu, Li
中央复合体是果蝇大脑中的一个突出结构。在飞行模拟器中对大脑经过基因改造的果蝇进行的视觉学习实验表明，其子结构之一（扇形身体）中的两组水平神经元是果蝇视觉模式记忆所必需的。然而，人们对视觉模式记忆中央复合体的其他成分的作用知之甚少。在这里，我们表明，椭圆体中的一小部分神经元（中央复合体的另一个子结构并与扇形体相连）也是视觉模式记忆所必需的。芜菁甘蓝腺苷酸环化酶在扇形体或椭圆体中的局部表达足以挽救 rut(2080) 突变体的记忆缺陷。然后我们对芜菁甘蓝任一结构进行 RNA 干扰，发现它们都是视觉模式记忆所必需的。此外，我们在尺寸、轮廓方向和垂直紧凑度等多个视觉模式参数下测试了上述获救的果蝇，并揭示了扇形体和椭圆体在视觉模式记忆中的不同作用。我们的研究定义了果蝇视觉模式记忆中央复合体的复杂神经回路。
The central complex is a prominent structure in the Drosophila brain. Visual learning experiments in the flight simulator, with flies with genetically altered brains, revealed that two groups of horizontal neurons in one of its substructures, the fan-shaped body, were required for Drosophila visual pattern memory. However, little is known about the role of other components of the central complex for visual pattern memory. Here we show that a small set of neurons in the ellipsoid body, which is another substructure of the central complex and connected to the fan-shaped body, is also required for visual pattern memory. Localized expression of rutabaga adenylyl cyclase in either the fan-shaped body or the ellipsoid body is sufficient to rescue the memory defect of the rut(2080) mutant. We then performed RNA interference of rutabaga in either structure and found that they both were required for visual pattern memory. Additionally, we tested the above rescued flies under several visual pattern parameters, such as size, contour orientation, and vertical compactness, and revealed differential roles of the fan-shaped body and the ellipsoid body for visual pattern memory. Our study defines a complex neural circuit in the central complex for Drosophila visual pattern memory.