Liposome behavior in capillary electrophoresis.
Liposome behavior in capillary electrophoresis.
复制标题
毛细管电泳中的脂质体行为。
作者：
M. Roberts;L. Locascio;W. Maccrehan;R. Durst
研究了毛细管电泳中脂质体的行为，目的是开发一种潜在的方法来表征制备的用于工业和分析应用的脂质体。本研究表征了脂质体在不同条件下的电泳行为，以提供有关电泳迁移率和脂质体-毛细管表面相互作用的信息。将该方法的结果与传统激光光散射方法的结果进行了比较，以获得脂质体制剂的尺寸信息。此外，脂质体和表面活性剂n-辛基-β-d-葡萄糖吡喃苷的反应在散装溶液实验中离线进行，在毛细管中在线进行。通过多次电动注射自动输送裂解剂被证明是诱导脂质体和其他试剂之间毛细管上反应的一般方法。此外，一些初步证据的使用脂质体作为一种疏水分配介质的分析分离提出。
The behavior of liposomes in capillary electrophoresis is studied for the purpose of developing a potential method for characterizing liposomes prepared for use in industrial and analytical applications. This study characterizes the electrophoretic behavior of liposomes under various conditions to provide information about electrophoretic mobility and liposome-capillary surface interactions. The results of this method are compared with the results obtained using traditional laser light-scattering methods to obtain size information about liposome preparations. Additionally, reactions of liposomes and the surfactant n-octyl-β-d-glucopyranoside are performed off-line in bulk solution experiments and on-line in the capillary. Automated delivery of lysis agents by multiple electrokinetic injections is demonstrated as a general method for inducing on-capillary reactions between liposomes and other reagents. Furthermore, some preliminary evidence on the use of liposomes as a hydrophobic partitioning medium for analytical separations is presented.