Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses
Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses
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II 系昆虫特异性黄病毒与致病性蚊媒黄病毒相比的血清学特征
DOI：
10.1016/j.bbrc.2022.05.080
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发表时间：
2022
影响因子：
3.1
通讯作者：
Orba Yasuko
中科院分区：
文献类型：
--
作者：
Tabata Koshiro;Itakura Yukari;Toba Shinsuke;Uemura Kentaro;Kishimoto Mai;Sasaki Michihito;Harrison Jessica J.;Sato Akihiko;Hall William W.;Hall Roy A.;Sawa Hirofumi;Orba Yasuko
黄病毒属包括病原性蜱媒和蚊媒黄病毒以及非病原性昆虫特异性黄病毒（ISFV）。基于全氨基酸序列的系统发育分析表明，谱系II ISFV与致病性黄病毒具有相似性。在这项研究中，我们使用的免疫血清的反应性分析与Psorophora黄病毒（PSFV）作为一个谱系IIa ISFV，和巴克吉病毒（BJV）作为一个谱系IIb ISFV，以评估抗原的相似性之间的谱系IIa和IIb ISFV，和致病性蚊媒黄病毒（MBFV）。结合和抗体依赖性增强试验表明，抗PSFV血清与MBFV抗原具有广泛的交叉反应性，而抗BJV血清具有低交叉反应性。很少观察到两种II系ISFV抗血清都能中和MBFV。这些结果表明，谱系IIa ISFV PSFV比谱系IIb ISFV BJV与MBFV具有更多的抗原相似性。
The genusFlavivirusincludes pathogenic tick- and mosquito-borne flaviviruses as well as non-pathogenic insect-specific flaviviruses (ISFVs). Phylogenetic analysis based on whole amino acid sequences has indicated that lineage II ISFVs have similarities to pathogenic flaviviruses. In this study, we used reactive analysis with immune serum against Psorophora flavivirus (PSFV) as a lineage IIa ISFV, and Barkeji virus (BJV) as a lineage IIb ISFV, to evaluate the antigenic similarity among lineage IIa and IIb ISFVs, and pathogenic mosquito-borne flaviviruses (MBFVs). Binding and antibody-dependent enhancement assays showed that anti-PSFV sera had broad cross-reactivity with MBFV antigens, while anti-BJV sera had low cross-reactivity. Both of the lineage II ISFV antisera were rarely observed to neutralize MBFVs. These results suggest that lineage IIa ISFV PSFV has more antigenic similarity to MBFVs than lineage IIb ISFV BJV.