Hawaiian larval stomatopods: molecular and morphological diversity
Hawaiian larval stomatopods: molecular and morphological diversity
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夏威夷口足类幼虫：分子和形态多样性
DOI：
10.11646/zootaxa.5214.2.5
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发表时间：
2022
期刊：
影响因子：
0.9
通讯作者：
PORTER, MEGAN L.
中科院分区：
文献类型：
--
作者：
STECK, MIREILLE;WINNICKI, ELIZABETH;KOBAYASHI, DONALD R.;WHITNEY, JONATHAN L.;AHYONG, SHANE T.;PORTER, MEGAN L.
单用形态学来估计口足类物种多样性长期以来一直很困难;虽然已经描述了450多个物种，但尽管成虫的神秘行为，新物种仍在定期被发现。然而，口足类的幼虫更容易获得，由于他们的远洋栖息地，并已成为最近的多样性研究的重点。形态多样性的研究描述了幼虫口足类的保守和分歧的特征，但通常不能与特定的物种。相反，使用DNA条形码的口足类幼虫的遗传研究可用于估计物种多样性，但通常不与已知物种的形态特征分析。在这里，我们联合收割机这两种方法，幼虫形态学和遗传学，估计口足类物种多样性在夏威夷群岛。超过22个操作分类单位（OTU）的基因，对应于20个特征的形态类型。鉴定了口足类三个主要超科的物种：虾蛄总科（4个OTU，3个形态型）、角虾蛄总科（9，8）和Lysiosquilloidea（6，7）。其中，与先前记录的夏威夷物种相比，基于幼虫形态型和OTU，Lysiosquilloids更多样化（3），而与先前记录的七个物种相比，虾蛄类的物种多样性较低，由收集的幼虫代表。两个OTU/形态型不能被鉴定为超科，因为它们的分子和形态特征与任何可用信息都不匹配，这表明它们属于采样不足的超科。拟虾蛄（Pseudosquillana richeri）是首次在夏威夷发现的虾蛄科鱼类。这项研究有助于更新估计夏威夷口足类动物的多样性，共24个记录的物种，提供参考，识别幼虫口足类动物在三个主要的超家庭，并强调缺乏知识的物种多样性更神秘的口足类动物超家庭，如Lysiosquilloidea。
Estimating stomatopod species diversity using morphology alone has long been difficult; though over 450 species have been described, new species are still being discovered regularly despite the cryptic behaviors of adults. However, the larvae of stomatopods are more easily obtained due to their pelagic habitat, and have been the focus of recent studies of diversity. Studies of morphological diversity describe both conserved and divergent traits in larval stomatopods, but generally cannot be linked to a particular species. Conversely, genetic studies of stomatopod larvae using DNA barcoding can be used to estimate species diversity, but are generally not linked to known species by analyses of morphological characters. Here we combine these two approaches, larval morphology and genetics, to estimate stomatopod species diversity in the Hawaiian Islands. Over 22 operational taxonomic units (OTUs) were identified genetically, corresponding to 20 characterized morphological types. Species from three major superfamilies of stomatopod were identified: Squilloidea (4 OTUs, 3 morphotypes), Gonodactyloidea (9, 8), and Lysiosquilloidea (6, 7). Among these, lysiosquilloids were more diverse based on larval morphotypes and OTUs as compared to previously documented Hawaiian species (3), while squilloids had a lower diversity of species represented by collected larvae as compared to the seven species previously documented. Two OTUs / morphotypes could not be identified to superfamily as their molecular and morphological features did not closely match any available information, suggesting they belong to poorly sampled superfamilies. The pseudosquillid, Pseudosquillana richeri, was discovered for the first time from Hawaiʻi. This study contributes an updated estimate for Hawaiian stomatopod diversity for a total of 24 documented species, provides references for identification of larval stomatopods across the three major superfamilies, and emphasizes the lack of knowledge of species diversity in more cryptic stomatopod superfamilies, such as Lysiosquilloidea.