Cortical projections of the non-entorhinal hippocampal formation in the cynomolgus monkey (Macaca fascicularis)
Cortical projections of the non-entorhinal hippocampal formation in the cynomolgus monkey (Macaca fascicularis)
复制标题
DOI：
10.1046/j.0953-816x.2001.01662.x
复制
发表时间：
2001-08-01
影响因子：
3.4
通讯作者：
Muñoz, M
中科院分区：
文献类型：
--
作者：
Insausti, R;Muñoz, M
情景记忆巩固需要大脑皮层和海马结构之间的解剖通路的完整性。虽然海马结构的最大皮质输出起源于内嗅皮质，但已报道了从CA 1、下托和前下托到皮质的直接投射。本研究的目的是评估这些预测的程度，地形和相对强度，作为记忆处理的平行/替代路线。在28只食蟹猴中共进行了45次注射。通过直接暴露皮质表面，在额叶、颞叶和顶叶的不同皮质区域以及扣带皮质中制备荧光示踪剂（20例，3%固蓝，2%二脒基黄）或1% WGA-HRP（8例）的皮质沉积物。适当的生存后，动物进行灌注和大脑连续切片在50 μ m和逆行标记绘制与X-Y数字化系统。逆行神经元标记观察CA 1，下托，前下托和旁下托;它是缺席的齿状回，CA 3和CA 2。与海马结构的其他部分相比，CA 1-下托边缘的标记神经元的数量最多（特别是在吻周皮层沉积后），其次是内侧额叶皮层，颞极，眶额，前和后扣带皮层，顶叶和颞下皮层，后颞下和外侧额叶皮层后无标记。我们的研究结果表明，CA 1，下托，前下托和旁下托发送直接输出到皮层区。这种非内嗅的海马结构皮质输出可能与记忆处理有关。
Episodic memory consolidation requires the integrity of the anatomical pathways between the cerebral cortex and the hippocampal formation. Whilst the largest cortical output of the hippocampal formation originates in the entorhinal cortex, direct projections from CA1, subiculum and presubiculum to the cortex have been reported. The aim of this study is the assessment of the extent, topography and relative strength of those projections, as a parallel/alternate route of memory processing. A total of 45 injections in 28 Macaca fascicularis monkeys were used. Cortical deposits of fluorescent tracers (20 cases, 3% Fast Blue, 2% Diamidino Yellow) or 1% WGA-HRP (eight cases) were made in different cortical areas of the frontal, temporal and parietal lobes, as well as cingulate cortex by direct exposure of the cortical surface. After appropriate survival, animals were perfused and the brains serially sectioned at 50 mum and the retrograde labelling charted with an X-Y digitizing system. Retrograde neuronal labelling was observed in CA1, subiculum, presubiculum and parasubiculum; it was absent in the dentate gyrus, CA3 and CA2. Compared to other portions of the hippocampal formation, the CA1-subiculum border had the highest number of labelled neurons (especially after deposits in the rostral perirhinal cortex), followed by medial frontal cortex, temporal pole, orbitofrontal, anterior and posterior cingulate cortices, parietal and inferotemporal cortices, and no labelling after posterior inferotemporal and lateral frontal cortices. Our results indicate that CA1, subiculum, presubiculum and parasubiculum send direct output to cortical areas. This nonentorhinal, hippocampal formation cortical output may be relevant in memory processing.