Structure-Function Correlations of Poly(Amido Amine)s for Gene Delivery
Structure-Function Correlations of Poly(Amido Amine)s for Gene Delivery
复制标题
用于基因传递的聚（酰胺基胺）的结构-功能相关性
DOI：
10.1002/mabi.201600297
复制
发表时间：
2017
影响因子：
4.6
通讯作者：
Ding Pingtian
中科院分区：
文献类型：
--
作者：
Sun Yanping;Xian Lei;Yu Jiankun;Yang Tianzhi;Zhang Jinmin;Yang Zhen;Jiang Jingzheng;Cai Cuifang;Zhao Xiaoyun;Yang Li;Ding Pingtian
聚胺胺（PAAs）的多功能性在阶梯聚合物中几乎是独一无二的。不同的官能团可以很容易地引入到这些聚合物中，以增加功能，如细胞内在化、电荷位移、生物可延展性、“隐身”特性和靶向部分，同时保持这些聚合物的整体结构完整性。聚胺胺是一个独特的研究平台，以阐明其复杂的结构-功能关系。研究表明，胍基、羧基、二硫键、烷基链、支链、乙酰基、苯甲酰和季烟酰胺基团可以影响基因传递的许多步骤，如DNA凝聚、细胞摄取、内体逃逸、核进入和最终的基因表达。作者系统地讨论了PAAs基因传递的结构-功能相关性，并阐述了如何通过改变聚合物的结构来调节聚合物的性能。
Poly(amido amine)s' (PAAs) versatility are nearly unique among stepwise polymers. Different functional groups can be easily introduced into these polymers to add functionality such as cell internalization, charge‐shift, bioreducibility, “stealth” properties, and targeting moieties, while maintaining the bulk structural integrity of these polymers. The poly(amido amine)s are used as a unique research platform to elucidate their complex structure–function relationship. It is shown that guanidinium group, carboxyl group, disulfide bond, alkyl chain, branching, acetyl groups, benzoyl groups, and quaternary nicotinamide moieties can influence many steps of gene delivery, such as DNA condensation, cellular uptake, endosomal escape, nuclear entry, and finally gene expression. The authors systematically discuss the structure–function correlations of PAAs for gene delivery, and elaborate how the properties of polymers can be adjusted by changing the polymeric structure.