Association of long non-coding RNA MEG3 polymorphisms with oral squamous cell carcinoma risk
Association of long non-coding RNA MEG3 polymorphisms with oral squamous cell carcinoma risk
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长链非编码RNA MEG3多态性与口腔鳞状细胞癌风险的关联
DOI：
10.1111/odi.13103
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发表时间：
2019-07-01
期刊：
影响因子：
3.8
通讯作者：
Yuan, Hua
中科院分区：
文献类型：
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作者：
Hou, Yunwen;Zhang, Bo;Yuan, Hua
目的长链非编码RNA（lncRNA）MEG 3与口腔鳞状细胞癌（OSCC）等多种肿瘤相关。单核苷酸多态性（SNPs）可能通过改变lncRNA的功能而影响癌症风险。但关于SNPs与MEG 3在肿瘤中的关系的研究较少。因此，我们推测MEG 3基因的SNPs可能影响口腔鳞癌的发病风险。材料与方法对1,428名受试者（444名OSCC患者和984名无癌对照）进行MEG 3中所选SNPs的基因分型。Logistic回归分析MEG 3基因SNPs与口腔鳞癌发病风险的关系。通过荧光素酶活性测定探讨SNPs的功能。结果rs 11160608与口腔鳞癌的发病风险有显著性差异（显性模型：校正OR = 1.36，95%CI = 1.06-1.76，p = 0.017）。此外，rs 11160608 CC基因型与OSCC风险的影响在饮酒者中比非饮酒者强得多（校正OR = 1.79，95%CI = 1.17-2.73，p = 0.007）。报告基因检测结果显示，rs 11160608 A等位基因的荧光素酶活性低于rs 11160608 C等位基因。结论MEG 3基因rs 11160608位点可能通过介导miRNA的结合在口腔鳞癌中发挥重要作用。
Objective Long non-coding RNA (lncRNA) MEG3 was associated with multiple types of cancers such as oral squamous cell carcinoma (OSCC). Single nucleotide polymorphisms (SNPs) might affect cancer risk by modifying the function of lncRNAs. But fewer study researched the relationship between SNPs and MEG3 in cancers. Considering these reasons, we supposed SNPs in MEG3 may influence the risk of OSCC. Material and Methods The selected SNPs in MEG3 were genotyped in 1,428 subjects (444 patients with OSCC and 984 cancer-free controls). The relationship between SNPs in MEG3 and OSCC risk was calculated by logistic regression analysis. The function of the SNPs was explored by luciferase activity assay. Results A statistically significant increased risk was observed between rs11160608 and OSCC (Dominant model: adjusted OR = 1.36, 95% CI = 1.06-1.76, p = 0.017). Moreover, the effect of rs11160608 CC genotype with OSCC risk was much strong in drinkers than non-drinkers (adjusted OR = 1.79, 95% CI = 1.17-2.73, p = 0.007). Furthermore, the luciferase activity of rs11160608 A allele was lower than rs11160608 C allele by luciferase reporter assay. Conclusion Our study confirmed that the SNP rs11160608 in MEG3 might play an important role in OSCC by interring the binding of miRNA.