Light-induced rapid Ca2+ response and MAPK phosphorylation in the cells heterologously expressing human OPN5
Light-induced rapid Ca2+ response and MAPK phosphorylation in the cells heterologously expressing human OPN5
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异源表达人 OPN5 的细胞中光诱导的快速 Ca2 反应和 MAPK 磷酸化
DOI：
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发表时间：
2014
影响因子：
4.6
通讯作者：
Takeo Takahashi
中科院分区：
文献类型：
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作者：
T. Sugiyama;Hirobumi Suzuki;Takeo Takahashi
Molecular imaging is a powerful tool for investigating intracellular signalling, but it is difficult to acquire conventional fluorescence imaging from photoreceptive cells. Here we demonstrated that human opsin5 (OPN5) photoreceptor mediates light-induced Ca2+ response in human embryonic kidney (HEK293) and mouse neuroblastoma (Neuro2a) cell lines using a luminescence imaging system with a fluorescent indicator and a newly synthesized bioluminescent indicator. Weak light fluorescence and bioluminescence imaging revealed rapid and transient light-stimulated Ca2+ release from thapsigargin-sensitive Ca2+ stores, whereas long-lasting Ca2+ elevation was observed using a conventional fluorescence imaging system. Bioluminescence imaging also demonstrated that OPN5 activation in HEK293 cells induced a decrease in pertussis toxin–sensitive cAMP, confirming previous reports. In addition, ultraviolet radiation induced the phosphorylation of mitogen-activated protein kinases when OPN5 was stimulated in Neuro2a cells. These findings suggest that the combination of these imaging approaches may provide a new means to investigate the physiological characteristics of photoreceptors.
影响因子：
4.7
作者：
Tsigos, C;Chrousos, GP
通讯作者：
Chrousos, GP
丝裂原激活蛋白激酶有助于脂多糖诱导的下丘脑室旁核中促肾上腺皮质激素释放激素合成神经元的激活。
影响因子：
4.8
作者：
Singru,PrafulS;Sanchez,Edith;Acharya,Runa;Fekete,Csaba;Lechan,RonaldM
通讯作者：
Lechan,RonaldM