Macrophage-mediated inhibitory effect of Zingiber officinale Rosc, a traditional Oriental herbal medicine, on the growth of influenza A/Aichi/2/68 virus
Macrophage-mediated inhibitory effect of Zingiber officinale Rosc, a traditional Oriental herbal medicine, on the growth of influenza A/Aichi/2/68 virus
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DOI：
10.1142/s0192415x06003722
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发表时间：
2006-01-01
影响因子：
5.7
通讯作者：
Ochiai, H
中科院分区：
文献类型：
--
作者：
Imanishi, N;Andoh, T;Ochiai, H
研究了东方中草药--铁皮石斛（ZOR）对A/Aichi/2/68（Aichi）流感病毒在犬肾细胞（MDCK）中的抑制作用。将ZOR（0.1类似于100 μ g/ml）直接加入到感染的细胞中没有任何抑制作用。然而，ZOR诱导的RAW细胞（小鼠巨噬细胞（My）细胞系）的条件培养基（ZOR-CM）对MDCK细胞表现出明显的抑制作用，而无细胞毒性。与ZOR-CM的时间依赖性抑制作用一致，已经证明，通过在ZOR刺激的RAW细胞中诱导TNF-α mRNA表达，肿瘤坏死因子（TNF）-α在ZOR-CM中逐渐积累。相反，ZOR-CM的抑制作用显著降低后，去除TNF-α的免疫复合物与抗TNF-α单克隆抗体的形成。这些数据表明，ZOR本身对流感病毒的生长没有抑制作用，但可以通过导致TNF-α产生的巨噬细胞活化来发挥其作用。
The inhibitory effect of Zingiber officinale Rosc (ZOR), an Oriental traditional herbal medicine, on the growth of influenza A/Aichi/2/68 (Aichi) virus was investigated in Madin-Darby canine kidney (MDCK) cells. Direct addition of ZOR (0.1 similar to 100 mu g/ml) to the infected cells did not have any inhibitory effect. However, the ZOR-induced conditioned medium (ZOR-CM) of RAW cells, a murine macrophage (My) cell line, exhibited an apparent inhibitory effect on MDCK cells without cytotoxicity. In accordance with the time-dependent inhibitory effect of ZOR-CM, it has been demonstrated that tumor necrosis factor (TNF)-alpha was gradually accumulated in ZOR-CM by the induction of TNF-alpha mRNA expression in ZOR-stimulated RAW cells. Conversely, the inhibitory effect of ZOR-CM was reduced significantly by the removal of TNF-alpha after the formation of an immune complex with anti-TNF-alpha monoclonal antibody. These data suggested that ZOR itself has no inhibitory effect on the growth of influenza virus, but could exert its effect via macrophage activation leading to production of TNF-alpha.