Transmission networks of HIV-1 among men having sex with men in the Netherlands
Transmission networks of HIV-1 among men having sex with men in the Netherlands
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DOI：
10.1097/qad.0b013e328333ddee
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发表时间：
2010-01-01
期刊：
影响因子：
3.8
通讯作者：
de Wolf, Frank
中科院分区：
文献类型：
--
作者：
Bezemer, Daniela;van Sighem, Ard;de Wolf, Frank
目的：深入了解荷兰男男性行为者 (MSM) 中的 HIV-1 传播网络。设计：根据作为荷兰艾滋病治疗评估 (ATHENA) 全国观察队列的一部分进行监测的 2877 名 HIV-1 B 亚型感染患者分离的聚合酶序列构建系统发育树。方法：对于已知感染日期的 MSM，最 当相似的序列以至少 99% 的 bootstrap 值聚类时，它们被选择作为潜在的传输对。从感染到继续传播的时间被估计为每个传播对的感染日期之间的中位时间。使用整个系统发育树追踪耐药菌株的感染源。结果：在 1987 年至 2007 年间已知感染日期的 403 个 MSM 的序列中，175 个 (43%) 形成 63 个簇。继续传播的中位时间为 1.4 年（四分位距 0.6-2.7）。 24 名 (6%) MSM 携带带有耐药性相关突变的病毒，其中 13 名与整个系统发育树中其他 28 名患者的序列一起分在 8 个簇中。六个簇包含从 29 名男性获得的序列，全部呈现相同的耐药相关突变。结论：从我们对可能的传播对的选择，我们得出结论，荷兰受感染的 MSM 的 HIV-1 的进一步传播发生在初次感染期间和之后。抗逆转录病毒治疗人群中耐药菌株的传播有限，但具有耐药相关突变的菌株已形成亚流行。 (C) 2010 年 Wolters Kluwer Health 垂直条 Lippincott Williams & Wilkins
Objective: To obtain insight in the HIV-1 transmission networks among men having sex with men (MSM) in the Netherlands.Design: A phylogenetic tree was constructed from polymerase sequences isolated from 2877 HIV-1 subtype B-infected patients monitored as part of the AIDS Therapy Evaluation in the Netherlands (ATHENA) nationwide observational cohort.Methods: For MSM with a known date of infection, the most similar sequences were selected as potential transmission pairs when they clustered with bootstrap value of at least 99%. Time from infection to onward transmission was estimated as the Median time between dates of infection for each transmission pair. The source of infections with a resistant strain was traced using the entire phylogenetic tree.Results: Of sequences from 403 MSM with a known date of infection between 1987 and 2007, 175 (43%) formed 63 clusters. Median time to onward transmission was 1.4 years (interquartile range 0.6-2.7). Twenty-four (6%) MSM carried a virus with resistance-related mutations, 13 of these were in eight clusters together with sequences from 28 other patients in the entire phylogenetic tree. Six clusters contained sequences obtained from 29 men all presenting the same resistance-related mutations.Conclusion: From our selection of likely transmission pairs, we conclude that onward transmission of HIV-1 from infected MSM in the Netherlands happens both during and after primary infection. Transmission of resistant strains from the antiretroviral therapy-treated population is limited, but strains with resistance-related mutations have formed subepidemics. (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins