Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH.
Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH.
复制标题
ACTH 调节牛肾上腺皮质 NADPH-细胞色素 P-450 还原酶的合成和活性。
DOI：
10.1016/0006-291x(85)90095-6
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发表时间：
1985
影响因子：
3.1
通讯作者：
Waterman,MR
中科院分区：
文献类型：
--
作者：
Dee,A;Carlson,G;Smith,C;Masters,BS;Waterman,MR
牛肾上腺皮质细胞的原代单层培养已被用于证明ACTH调节NADPH-细胞色素P-450还原酶的合成和活性。诱导的时间模式与先前报道的细胞色素P-450 17α和P-450 C21（由P-450还原酶提供服务的微粒体类固醇羟化酶）的时间模式相关。体外翻译研究的结果表明，促肾上腺皮质激素调节P-450还原酶的合成在转录水平上，并显示，肾上腺皮质酶合成的成熟形式。ACTH诱导肾上腺皮质中P-450还原酶合成的作用可被双丁酰cAMP模拟，但不能被诱导肝脏中P-450还原酶合成的苯巴比妥模拟。
Primary, monolayer cultures of bovine adrenocortical cells have been utilized to demonstrate that ACTH regulates the synthesis and thus the activity of NADPH-cytochrome P-450 reductase. The temporal pattern of induction correlates well with that previously reported for cytochromes P-450 17α and P-450 C21, microsomal steroid hydroxylases serviced by P-450 reductase. Results of in vitro translation studies suggest that ACTH regulates P-450 reductase synthesis at the transcriptional level and show that the adrenocortical enzyme is synthesized as the mature form. The action of ACTH to induce P-450 reductase synthesis in the adrenal cortex can be mimicked by dibutyryl cAMP, but not by phenobarbital which induces P-450 reductase synthesis in liver.