Determination of β-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection
Determination of β-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection
复制标题
DOI：
10.1016/j.meatsci.2010.01.018
复制
发表时间：
2010-06-01
期刊：
影响因子：
7.1
通讯作者：
Ye, Jiannong
中科院分区：
文献类型：
--
作者：
Wang, Weiyu;Zhang, Yulian;Ye, Jiannong
采用毛细管电泳-电化学检测法（CE-ED）对盐酸克仑特罗、美托洛尔、莱克多巴胺、异氧舒普林和沙丁胺醇5种β-受体激动剂进行了快速分离测定。对影响分离和检测的因素进行了考察和优化。在最佳条件下，100 mmoL H_3BO_3-Na_2B_4O_7（pH9.0）运行缓冲液中，14 kV分离电压下，15 min内5种β-受体激动剂可得到良好分离。该方法已成功应用于猪饲料、猪尿液和猪肝中盐酸克伦特罗、美托洛尔、莱克多巴胺、异舒普林和沙丁胺醇的分析，为食品动物促生长β受体激动剂的非法使用提供了有效的监测手段。(C)2010爱思唯尔有限公司版权所有。
The fast separation capability of a capillary electrophoresis with electrochemical detection (CE-ED) system was demonstrated by determining five beta-agonists including clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in several real samples. Factors influencing the separation and detection were examined and optimized. Under the optimum conditions, the five beta-agonists could be well separated within 15 min at a separation voltage of 14 kV in a 100 mmoL H3BO3-Na2B4O7 running buffer (pH 9.0). This method was successfully used in the analysis of clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in pig feed, pig urine and pig liver, providing a useful method for monitoring the illegal use of beta-agonists from growth promotion in food-producing animals. (C) 2010 Elsevier Ltd. All rights reserved.