Comparison of diagnosis techniques for the protozoan parasite Bonamia ostreae in flat oyster Ostrea edulis
Comparison of diagnosis techniques for the protozoan parasite Bonamia ostreae in flat oyster Ostrea edulis
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DOI：
10.1016/j.aquaculture.2006.05.014
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发表时间：
2006-12-11
期刊：
影响因子：
4.5
通讯作者：
Figueras, Antonio
中科院分区：
文献类型：
--
作者：
Balseiro, Pablo;Fernandez Conchas, Ramon;Figueras, Antonio
近年来，欧洲扁牡蛎的养殖产量急剧下降，部分原因是原生动物寄生虫Bonamia ostrae，Bonamiosis的病原体。本文报道了PCR诊断B。用OIE（Office International des Epizooties）和欧盟推荐的两种经典的诊断方法，即组织学和细胞学方法，对20只牡蛎进行了比较。同一样品在两个不同实验室进行分析，结果表明，PCR方法具有较高的灵敏度和良好的实验室之间的相关性。这种方法比组织病理学和细胞学更便宜，更快，不需要经过专门培训的人员进行诊断。适用于大量牡蛎的快速筛选。(c)2006 Elsevier B.V保留所有权利。
In recent years, the cultured production of the flat oyster Ostrea edulis has suffered a dramatic decrease in Europe partially attributed to the protozoan parasite Bonamia ostreae, the causative agent of the Bonamiosis. In this paper the results of a PCR assay for the diagnosis of B. ostreae were compared with those obtained using two classical methods of diagnosis recommended by the OIE (Office International des Epizooties) and the European Union, histology and cytology. The same samples were analyzed by two different laboratories, showing that the results obtained with the PCR method have high sensitivity and good correlation between laboratories. This method is cheaper and faster than histopathology and cytology, with no need of specifically trained personnel to perform the diagnoses. It is appropriate for fast screening of stocks of great numbers of oysters. (c) 2006 Elsevier B.V All rights reserved.