Optimization of the production of hCG beta-like material by CaSki human cervical carcinoma cells in roller bottle culture.
Optimization of the production of hCG beta-like material by CaSki human cervical carcinoma cells in roller bottle culture.
复制标题
CaSki 人宫颈癌细胞在滚瓶培养中优化 hCG β 样物质的生产。
作者:
Hussa,RO;Pattillo,RA;Ruckert,AC;Rinke,ML;Cortesi,JP;Fein,HG
This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCGβ-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal: (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.
登录
查看更多内容
DOI:
--
发表时间:
1981
期刊:
影响因子:
--
作者:
E. Friedberg;P. Hanawalt
通讯作者:
P. Hanawalt
DOI:
--
发表时间:
1986
期刊:
影响因子:
--
作者:
J. Biaglow;M. Varnes;E. P. Clark;E. Epp
通讯作者:
E. Epp
DOI:
10.1016/0360-3016(86)90209-9
发表时间:
1986
期刊:
International journal of radiation oncology, biology, physics
影响因子:
--
作者:
Chiu,SM;Friedman,LR;Xue,LY;Oleinick,NL
通讯作者:
Oleinick,NL
影响因子:
3.4
作者:
CLARK, EP;EPP, ER;BIAGLOW, JE
通讯作者:
BIAGLOW, JE
影响因子:
2.9
作者:
Blakely,WF;Ward,JF;Joner,EI
通讯作者:
Joner,EI