Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells
Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells
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DOI：
10.1016/j.toxicon.2009.05.002
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发表时间：
2009-09-01
期刊：
影响因子：
2.8
通讯作者：
Spicer, Leon J.
中科院分区：
文献类型：
--
作者：
Caloni, Francesca;Ranzenigo, Giovanni;Spicer, Leon J.
镰刀菌属真菌毒素，如镰刀菌烯和玉米赤霉烯酮，是由霉菌产生的，并污染世界各地的各种谷物和饲料。镰刀菌属真菌的毒素包括镰刀菌烯、脱氧雪腐镰刀菌烯醇和T-2毒素（17）以及玉米赤霉烯酮，并与猪的繁殖性能差有关。然而，T2毒素对卵巢的直接影响尚未报道。因此，将来自小卵泡（1-5 mm）的猪颗粒细胞（GC）在5%胎牛血清和5%含猪血清的培养基中培养2天，然后在含各种剂量FSH、胰岛素样生长因子-I和T2（各种剂量/组合）的无血清培养基中培养2天，以评价T-2对类固醇产生和细胞增殖的影响。1、3、30和300 ng/mL的T2完全抑制FSH + IGF-I诱导的雌二醇产生，而0.3 ng/mL的T2抑制雌二醇产生40%。孕酮产生对T-2的抑制作用不太敏感，0.3 ng/mL没有作用，1 ng/mL仅抑制孕酮产生30%。在30和300 ng/mL T-2完全抑制FSH加IGF-I诱导的孕酮产生。还评价了12对IGF-I（0、3、10和30 ng/mL）剂量反应的影响; T2减弱了3-30 ng/mL IGF-I对类固醇产生和细胞增殖的刺激作用。12天后，血清诱导的颗粒细胞增殖降低了40%（P < 0.05），2天后降低了56%（P < 0.05）。目前的研究表明，第一次，72可能能够改变卵泡内的颗粒层的生长，除了它们对类固醇生成的影响。总之，U对颗粒细胞增殖和类固醇生成具有有效的直接剂量依赖性作用。这些直接的卵巢效应可能是饲料中污染镰刀菌毒素影响猪繁殖性能的一种机制。(C)2009爱思唯尔有限公司保留所有权利。
Fusarium mycotoxins, such as trichothecenes and zearalenone, are produced by molds and contaminate a large variety of grains and feedstuffs worldwide. Mycotoxins of Fusarium fungi include the trichothecenes, deoxynivalenol and T-2 toxin (17), and zearalenone, and have been implicated in poor reproductive performance in pigs. However, direct ovarian effects of T2 toxin have not been reported. Therefore, porcine granulosa cells (GC) from small follicles (1-5 mm) were cultured for 2 days in 5% fetal bovine serum and 5% porcine serum-containing medium followed by 2 days in serum-free medium containing various doses of FSH, insulin-like growth factor-I and T2 (at various doses/combinations) to evaluate the influence of T-2 on steroid production and cell proliferation. T2 at 1, 3, 30 and 300 ng/mL completely inhibited FSH plus IGF-I-induced estradiol production, whereas 0.3 ng/mL of T2 inhibited estradiol production by 40%. Progesterone production was less sensitive to the inhibitory effects of T-2 with 0.3 ng/mL having no effect and 1 ng/mL inhibiting progesterone production by only 30%. At 30 and 300 ng/mL T-2 completely inhibited FSH plus IGF-I-induced progesterone production. The impact of 12 on the dose-response to IGF-I (0, 3, 10 and 30 ng/mL) was also evaluated; T2 blunted the stimulatory effect of 3-30 ng/mL of IGF-I on steroid production and cell proliferation. Serum-induced granulosa cell proliferation was decreased (P < 0.05) by 40% after 1 day and by 56% after 2 days of 12 treatment. The present studies indicate for the first time that 72 may be able to alter in growth of the granulosa layer within ovarian follicles in addition to their effect on steroidogenesis. In conclusion, U has potent direct dose-dependent effects on granulosa cell proliferation and steroidogenesis. These direct ovarian effects could be one mechanism whereby contaminating Fusarium mycotoxins in feedstuffs could impact reproductive performance in swine. (C) 2009 Elsevier Ltd. All rights reserved.