Effects of Docosahexaenoic Acid on Chemokine Expression in Human Conjunctival Fibroblasts
Effects of Docosahexaenoic Acid on Chemokine Expression in Human Conjunctival Fibroblasts
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DOI：
10.1080/02713683.2019.1648832
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发表时间：
2020-01
影响因子：
2
通讯作者：
Hiroyuki Yazu;K. Fukagawa;N. Okada;H. Fujishima
中科院分区：
文献类型：
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作者：
Hiroyuki Yazu;K. Fukagawa;N. Okada;H. Fujishima
摘要目的：我们评估了人结膜成纤维细胞对炎症反应的趋化因子的产生以及ω-3脂肪酸对趋化因子表达的影响。方法原代培养的人结膜成纤维细胞与白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）共同孵育。研究了二十二碳六烯酸（DHA）预处理后Eotaxin-1和RANTES的表达。Western blotting检测DHA对核因子（NF）-κB（B）和信号转导和转录激活因子6（STAT 6）活化的影响。结果DHA预处理联合IL-4和TNF-α共刺激可显著抑制Eotaxin-1 mRNA的表达。RANTES的表达也同样受到抑制，但差异不显著。在DHA预处理的细胞中，Eotaxin-1和RANTES的分泌显著低于溶剂处理的细胞。Western blotting检测NF-κB和STAT 6蛋白表达，结果显示DHA预处理组NF-κB和STAT 6蛋白表达较溶剂对照组下调。结论DHA可用于治疗过敏性炎症。
ABSTRACT Purpose We assessed the production of chemokines by human conjunctival fibroblasts in response to inflammation and the effects of omega (ω)-3 fatty acids on chemokine expression. Methods Primary cultures of human conjunctival fibroblasts were incubated with interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-α). The expression of eotaxin-1 and RANTES in response to pretreatment with docosahexaenoic acid (DHA) was investigated. Moreover, western blotting was used to evaluate the effects of DHA on the activation of nuclear factor (NF)-κB and signal transducer and activator of transcription 6 (STAT6). Results The expression of eotaxin-1 mRNA was significantly suppressed by pretreatment with DHA with IL-4 and TNF-α costimulation. RANTES expression was similarly suppressed, but the difference was not significant. The secretion of eotaxin-1 and RANTES was significantly lower in DHA-pretreated cells than in vehicle-treated cells. Western blotting for NF-κB and STAT6 showed that these proteins were downregulated in the DHA pretreatment group compared with those in the vehicle control group. Conclusion The results of this study suggested that DHA could have applications in the management of allergic inflammation.