Hatching of cladoceran resting eggs: temperature and photoperiod
Hatching of cladoceran resting eggs: temperature and photoperiod
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DOI：
10.1111/j.1365-2427.2004.01312.x
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发表时间：
2005
影响因子：
2.7
通讯作者：
J. Vandekerkhove;S. Declerck;L. Brendonck;J. Conde-Porcuna;E. Jeppesen;L. Meester
中科院分区：
文献类型：
--
作者：
J. Vandekerkhove;S. Declerck;L. Brendonck;J. Conde-Porcuna;E. Jeppesen;L. Meester
摘要1.我们确定了温度和光周期条件下，45个枝孢属物种的孵化可以引起。识别适当的孵化线索是最重要的探索活动和滞育阶段之间的联系。2.孵化温度影响孵化成功的休眠蛋分离丹麦，比利时/荷兰和西班牙的沉积物。一般来说，大多数孵化和物种在15 ℃时被取回。丹麦和比利时/荷兰休眠蛋孵化更成功的长日照光周期下比连续照明。3.大多数物种可以检索孵化后的休眠蛋分离出的沉积物（0.4公斤）在一个单一的，精心选择的温度和光周期的组合。处理额外的沉积物样品下7个孵化制度只允许检测21%（西班牙）到34%（丹麦）更多的物种。4.孵化温度对休眠卵的孵化期有很大影响。我们的研究结果表明，孵化实验旨在评估枝角类物种丰富度，并在15 ° C进行，应持续至少2周的时间，之后可以从总的孵化组合中选择一个随机的子集的孵化（例如，n 1/4 100）。
SUMMARY 1. We identified temperature and photoperiod conditions under which the hatching of 45 cladoceran species could be elicited. Identification of appropriate hatching cues is of primary importance for the exploration of the ties between active and diapausing stages. 2. Incubation temperature affected the hatching success of resting eggs isolated from Danish, Belgian/Dutch and Spanish sediments. In general, most hatchlings and species were retrieved at 15 � C. Danish and Belgian/Dutch resting eggs hatched more successfully under a long day photoperiod than in continuous illumination. 3. Most species could be retrieved after incubation of resting eggs isolated from a limited amount of sediment (0.4 kg) under a single, well chosen combination of temperature and photoperiod. Processing additional sediment samples under seven more incubation regimes only allowed detection of 21% (Spain) to 34% (Denmark) more species. 4. The incubation period for resting eggs to hatch was strongly influenced by incubation temperature. Our results show that hatching experiments aimed at assessing cladoceran species richness and conducted at 15 � C should be continued for a period of at least 2 weeks, after which a random subset of hatchlings (e.g. n ¼ 100) can be selected from the total hatchling assemblage.