Metagenomic insights into microbial arsenic metabolism in shallow groundwater of Datong basin, China
Metagenomic insights into microbial arsenic metabolism in shallow groundwater of Datong basin, China
复制标题
中国大同盆地浅层地下水微生物砷代谢的宏基因组学研究
DOI：
10.1016/j.chemosphere.2019.125603
复制
发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Chuanyong Jing
中科院分区：
文献类型：
--
作者：
Liying Wang;Zhipeng Yin;Chuanyong Jing
地下水砷（As）含量升高是一个紧迫的环境问题，已导致中国大同盆地严重的地方病。砷的归宿和毒性通常受微生物砷代谢过程的调节。然而，人们对大同盆地的微生物群落和砷代谢知之甚少。本文采用宏基因组学方法对山阴县4口不同砷浓度水井的微生物群落结构和砷代谢基因进行了研究。结果表明，As的存在影响了微生物群落，红球菌属在高As浓度的井中显着富集。由于抗性基因从低砷到高砷的孔中占主导地位，并且砷外排基因如asarsBandacr3与砷浓度呈正相关，这表明微生物倾向于将砷泵出细胞作为砷解毒的策略。其他环境因素，包括氧化还原电位（ORP）、总有机碳（TOC）、硫酸盐和温度也在塑造微生物群落结构和砷代谢过程中发挥了作用。
Elevated arsenic (As) in groundwater is an urgent environmental problem that has caused serious endemic diseases in Datong basin, China. The fate and toxicity of As are generally regulated by microbial As metabolic processes. However, little is known about the microbial community and As metabolism in Datong basin. Herein, the microbial community structure and As metabolism genes in four wells with different levels of As concentration in Shanyin county were investigated using metagenomics approach. The results showed that the presence of As influenced the microbial communities, andRhodococcusgenus was significantly enriched in elevated As wells. As resistance genes were dominant from low to high As containing wells, and As efflux genes such asarsBandacr3were positively correlated with As concentrations, suggesting that microbes tend to pump As out of the cell as a strategy for As detoxification. Other environmental factors including oxidation-reduction potential (ORP), total organic carbon (TOC), sulfate, and temperature also played a role in shaping the microbial community structure and As metabolic processes.