Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole
Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole
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DOI：
10.1073/pnas.0807660105
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发表时间：
2008-10-07
影响因子：
11.1
通讯作者：
Blobel, Guenter
中科院分区：
文献类型：
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作者：
Wong, Richard W.;Blobel, Guenter
精确的有丝分裂染色体分离依赖于微管为基础的双极纺锤体的形成。我们报告，在有丝分裂纺锤体极的染色体亚基1（SMC 1）的粘附素亚基结构维护被招募到微管结合的RNA输出因子1（Rae 1）。我们将Rae 1结合位点定位于21个残基长的区域SMC 1（947-967），并提供了几条证据表明SMC 1的Ser（957）和Ser（966）磷酸化刺激Rae 1结合。这些组装途径的不平衡导致多极纺锤体的形成。我们的数据表明，粘附素对有丝分裂和间期细胞中染色单体的已知捆绑功能延伸到纺锤体极的微管。
Accurate mitotic chromosome segregation depends on the formation of a microtubule-based bipolar spindle apparatus. We report that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We locate the Rae1-binding site to a 21-residue-long region, SMC1(947-967) and provide several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.