Effect of selenium-sulfur interaction on the anabolism of sulforaphane in broccoli
Effect of selenium-sulfur interaction on the anabolism of sulforaphane in broccoli
复制标题
硒-硫相互作用对西兰花中萝卜硫素合成代谢的影响。
DOI：
10.1016/j.phytochem.2020.112499
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发表时间：
2020-11-01
期刊：
影响因子：
3.8
通讯作者：
Huang, Ke
中科院分区：
文献类型：
--
作者：
Mao, Shuxiang;Wang, Junwei;Huang, Ke
研究了S（以硫酸盐计）和Se（以亚硒酸盐计）处理（S mM/Se μ M：1/0、1/50、1/100、1/150、4/0、4/50、4/100和4/150）对萝卜硫素（一种抗癌化合物）的产生、其前体物质的积累以及与花椰菜中萝卜硫苷生物合成相关的基因的表达的影响。萝卜硫素产量和黑芥子酶活性显着增加与4 mM的S和100 μ M硒的组合应用在花椰菜。此外，萝卜硫素（萝卜硫素前体）和蛋氨酸（萝卜硫素底物）的浓度略有变化后硒的应用。进一步提高了硒-SMC的抗肿瘤活性。相关基因的表达分析表明，MY，编码黑芥子酶，硒处理强烈诱导。因此，硒处理诱导的黑芥子酶活性是影响萝卜硫素水解产萝卜硫素的主要因素。硒硫生物强化为高萝卜硫素、高抗癌硒化合物西兰花的培育提供了技术支撑。
The effects of S (as sulphate) and Se (as selenite) treatment (S mM/Se mu M: 1/0, 1/50, 1/100, 1/150, 4/0, 4/50, 4/100, and 4/150) on the production of sulforaphane (an anticancer compound), the accumulation of its precursor substance, and the expression of genes related to glucoraphanin biosynthesis in broccoli were examined. Sulforaphane yield and myrosinase activity increased significantly with the combined application of 4 mM S and 100 mu M Se on broccoli. Furthermore, the concentrations of glucoraphanin (a sulforaphane precursor) and methionine (a glucoraphanin substrate) slightly changed after Se application. And the strong anticancer activity of compound Se-SMC was further improved. Analysis of related gene expression showed that MY, which encodes myrosinase, was strongly induced by Se treatment. Thus, the myrosinase activity induced by Se treatment is the dominant factor affecting sulforaphane yield from glucoraphanin hydrolyzation. Selenium-sulfur biofortification provides a technical support for the cultivation of broccoli with high sulforaphane and high anti-cancer selenium compounds.