microRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression
microRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression
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DOI：
10.1111/cpr.12223
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发表时间：
2015-12-01
影响因子：
8.5
通讯作者：
Yun, Jun
中科院分区：
文献类型：
--
作者：
Li, Yongping;Huang, Ruixue;Yun, Jun
目的：miRNAs在人类肿瘤发生中发挥重要作用。本研究旨在检测miR-762在乳腺癌中的表达和功能。材料和方法：采用实时定量RT-PCR方法检测乳腺癌组织和乳腺癌细胞系(SK-BR-3、DA-MB-435s、MCF-7和MDA-MB-231、HBL-100)中miR-762的表达。我们恢复了miR-762在MCF-7细胞中的表达，以检测其功能。结果：miR-762在乳腺癌细胞株和乳腺癌细胞中均有高表达，其过表达促进了乳腺癌细胞的增殖和侵袭。干扰素调节因子7(IRF7)是miR-762的直接靶点，而miR-762的过表达降低了IRF7的表达。此外，IRF7被抑制，其水平与miR-762的表达呈负相关。结论：IRF7在人乳腺癌标本中具有靶向miR-762的肿瘤效应。
Objectives: miRNAs play crucial roles in human tumourigenesis. This study was performed to measure expression and function of miR-762 in breast cancer.Materials and methods: Expression of miR-762 in breast tissues and cell lines (SK-BR-3, DA-MB-435s, MCF-7 and MDA-MB-231, HBL-100) was measured by using real-time RT-PCR. We restored expression of miR-762 in MCF-7 cells to measure its functional roles. Luciferase assays were performed to reveal the target gene of miR-762.Results: Expression of miR-762 was high in both breast cancer cell lines and specimens, and its overexpression increased breast cancer cell proliferation and invasion. Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. Moreover, IRF7 was repressed, its levels inversely correlated to miR-762 expression. IRF7 rescued miR-762-induced cell invasion and proliferation.Conclusions: These results demonstrate that miR-762 tumour effect was achieved by targeting IRF7 in human breast cancer specimens.