Resting cells isobutanol production by Shimwellia blattae (p424IbPSO): Influence of growth culture conditions
Resting cells isobutanol production by Shimwellia blattae (p424IbPSO): Influence of growth culture conditions
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DOI：
10.1002/btpr.2705
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发表时间：
2018-09
影响因子：
2.9
通讯作者：
M. G. Acedos;V. E. Santos;F. García-Ochoa
中科院分区：
文献类型：
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作者：
M. G. Acedos;V. E. Santos;F. García-Ochoa
异丁醇是一种很有前途的汽油添加剂，甚至可以直接作为燃料使用。在这项工作中，生产异丁醇从葡萄糖Shimwellia blattae（p424 IbPSO）在静息细胞培养进行了研究。这种生产有两个阶段，涉及以前没有研究过的静息细胞阶段。在不同的操作条件下进行细胞生长：温度和培养基组成（YE、铵和IPTG浓度），寻找最高的异丁醇产量。此外，在三个不同的生长时间收集细胞，检查其异丁醇生产能力。最佳操作条件为：温度30°C，氮源为1.5 g L-1 YE和1.4 g L-1 NH 4+，诱导剂为0.5 mM IPTG。在早期生长时间收集的细胞明显更活跃。利用S.在静息细胞中使用p424 IbPSO是一种很好的策略，用于从葡萄糖生产异丁醇，产生比分批生长培养更好的结果，在最佳条件下，获得了60%的理论最大产量。此外，已经证明，如果细胞在较高温度和高IPTG浓度下培养，则在细胞质中形成包涵体，抑制静息细胞阶段的异丁醇产生。
Isobutanol is a promising gasoline additive and could even be a potential substitute used directly as combustible. In this work, the production of isobutanol from glucose by Shimwellia blattae (p424IbPSO) in resting cell cultures is studied. This production has two stages, involving a resting cell phase that has not been studied before. The cell growth was carried out under different operating conditions: temperature and medium composition (YE, ammonium, and IPTG concentrations), looking for the highest isobutanol production. Moreover, the cells were collected at three different growth times checking their isobutanol production capacity. The best operating conditions have been determined as: 30°C of temperature, a medium containing 1.5 g L−1 YE and 1.4 g L−1of ammonium as nitrogen sources, adding 0.5 mM IPTG as inducer. The cells collected at early growth times are significantly more active. The use of S. blattae (p424IbPSO) in resting cells is a good strategy for the production of isobutanol from glucose yielding better results than in batch growth cultures, a yield of 60% attainment of theoretical maximum yield is obtained under optimal conditions. In addition, it has been demonstrated that if the cells are cultured at higher temperatures and with high IPTG concentrations, inclusion bodies are formed in the cytoplasm inhibiting the isobutanol production in the resting cell stage.