Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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DOI：
10.3791/53974
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发表时间：
2016-06-01
影响因子：
1.2
通讯作者：
Nagase, Takahide
中科院分区：
文献类型：
--
作者：
Mikami, Yu;Matsuzaki, Hirotaka;Nagase, Takahide
纤维化常参与各种慢性进展性疾病的发病机制，如间质性肺疾病。病理特征是成纤维细胞灶的形成，这与疾病的严重程度有关。由成纤维细胞灶组成的间充质细胞被认为来自多种细胞来源，包括最初驻留在肺内的成纤维细胞和来自骨髓的循环纤维细胞。最近，间充质细胞经历了上皮-间充质转化(EMT)，也被认为参与了纤维化的发病机制。此外，转化生长因子β可诱导EMT，促炎症细胞因子如肿瘤坏死因子α可增强EMT。凝胶收缩试验是评价成纤维细胞收缩功能的理想体外模型，它是成纤维细胞特有的功能之一，有助于伤口修复和纤维化。在这里，凝胶收缩试验的发展被证明是为了评估接受EMT的间充质细胞的收缩能力。
Fibrosis is often involved in the pathogenesis of various chronic progressive diseases such as interstitial pulmonary disease. Pathological hallmark is the formation of fibroblastic foci, which is associated with the disease severity. Mesenchymal cells consisting of the fibroblastic foci are proposed to be derived from several cell sources, including originally resident intrapulmonary fibroblasts and circulating fibrocytes from bone marrow. Recently, mesenchymal cells that underwent epithelial-mesenchymal transition (EMT) have been also supposed to contribute to the pathogenesis of fibrosis. In addition, EMT can be induced by transforming growth factor beta, and EMT can be enhanced by pro-inflammatory cytokines like tumor necrosis factor alpha. The gel contraction assay is an ideal in vitro model for the evaluation of contractility, which is one of the characteristic functions of fibroblasts and contributes to wound repair and fibrosis. Here, the development of a gel contraction assay is demonstrated for evaluating contractile ability of mesenchymal cells that underwent EMT.