HPLC Determination of Strychnine and Brucine in Rat Tissues and the Distribution Study of Processed Semen Strychni
HPLC Determination of Strychnine and Brucine in Rat Tissues and the Distribution Study of Processed Semen Strychni
复制标题
高效液相色谱法测定大鼠组织中马钱子碱和马钱子碱的含量及炮制马钱子的分布研究
DOI：
10.1248/yakushi.131.721
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发表时间：
2011-05-01
影响因子：
0.3
通讯作者：
Cai, Bao-chang
中科院分区：
文献类型：
--
作者：
Chen, Jun;Hou, Ting;Cai, Bao-chang
建立了一种简便、低成本的高效液相色谱-紫外吸收检测法，用于同时测定马钱子炮制品中士的宁和马钱子碱在大鼠组织（肾、肝、脾、肺、心、胃、小肠、脑和血浆）中的含量。在HPLC之前，用简单的液-液萃取处理组织样品。对于不同组织或血浆样本，LOQ在0.039至0.050 μ g/ml范围内。萃取回收率为71.63 ~ 98.79%。线性范围为0.05 ~ 2 μ g/ml，相关系数大于0.991。日内和日间精密度小于15%。以马钱子炮制品为原料，经静脉注射1 mg/kg马钱子生物碱粗提物后，用该方法测定马钱子碱和士的宁的组织分布。结果表明士的宁和马钱子碱具有相似的组织分布特征。在肾脏中观察到最高水平，而在脑中观察到最低水平。表明肾脏可能是原型士的宁和马钱子碱的主要排泄器官。士的宁和马钱子碱难以通过血脑屏障。此外，士的宁和马钱子碱在大鼠组织中没有长期蓄积。
A simple and low-cost HPLC method with UV absorbance detection was developed and validated to simultaneously determine strychnine and brucine, the most abundant alkaloids in the processed Semen Strychni, in rat tissues (kidney, liver, spleen, lung, heart, stomach, small intestine, brain and plasma). The tissue samples were treated with a simple liquid-liquid extraction prior to HPLC. The LOQs were in the range of 0.039 similar to 0.050 mu g/ml for different tissue or plasma samples. The extraction recoveries varied from 71.63 to 98.79%. The linear range was 0.05-2 mu g/ml with correlation coefficient of over 0.991. The intra- and inter-day precision was less than 15%. Then the method was used to measure the tissue distribution of strychnine and brucine after intravenous administration of 1 mg/kg crude alkaloids fraction (CAF) extracted from the processed Semen Strychni. The results revealed that strychnine and brucine possessed similar tissue distribution characterization. The highest level was observed in kidney, while the lowest level was found in brain. It was indicated that kidney might be the primary excretion organ of prototype strychnine and brucine. It was also deduced that strychnine and brucine had difficulty in crossing the blood-brain barrier. Furthermore, no long-term accumulation of strychnine and brucine was found in rat tissues.