Sensitivity to dietary obesity linked to a locus on chromosome 15 in a CAST/Ei x C57BL/6J F2 intercross.
Sensitivity to dietary obesity linked to a locus on chromosome 15 in a CAST/Ei x C57BL/6J F2 intercross.
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CAST/Ei x C57BL/6J F2 杂交中对饮食肥胖的敏感性与 15 号染色体上的基因座有关。
DOI：
10.1007/s003359900204
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
West,DB
中科院分区：
文献类型：
--
作者：
York,B;Lei,K;West,DB
详细介绍了一种新的饮食依赖性多基因肥胖模型。CAST/Ei（Mus m. castaneus）小鼠在高脂肪（32 kcal%脂肪）饮食12周后仍保持苗条，而C57BL/ 6J小鼠则变得肥胖。f2群体中负责肥胖分离的基因来自CAST/Ei和C57BL/6J小鼠之间的交叉。通过对先前与肥胖相关的位点进行简单序列长度多态性（slps）的数量性状分析，在第15号染色体上发现了一个数量性状位点（QTL），该位点约占肥胖变异的9%，占肠系膜库大小变异的14%。该基因座似乎与先前在SWR/J × AKR/J杂交的f2群体中发现的控制体脂含量的膳食肥胖-3 （Do3）基因座位于同一位置。这也与在BSB小鼠中发现的多基因肥胖-4 （Mob4）位点相同，BSB小鼠表现出自发性多基因肥胖。在靠近Chr 2和Chr 7的单基因突变位点上也发现了暗含的连锁。
Details of a new model of diet-dependent polygenic obesity are presented. CAST/Ei(Mus m. castaneus)mice remain lean after 12 weeks on a high-fat (32 kcal% fat) diet, while C57BL/ 6J mice become obese. The genes responsible for the obesity segregate in an F2population derived from an intercross between CAST/Ei and C57BL/6J mice. Quantitative trait analysis, with simple sequence length polymorphisms (SSLPs) at loci previously linked to rodent obesities, identified a quantitative trait locus (QTL) on Chromosome (Chr) 15, accounting for approximately 9% of the variance in adiposity and 14% of the variance in mesenteric depot size. This locus appears to be at the same location as the dietary obesity-3 (Do3) locus controlling body fat content, which was previously identified in an F2population derived from an SWR/J × AKR/J cross. This is also at the same location as the multigenic obesity-4 (Mob4) locus found in BSB mice, which display spontaneous polygenic obesity. Suggestive linkage also was found at loci close to the single gene mutationsAyon Chr 2 andtubon Chr 7.