Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length
Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length
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DOI：
10.1016/j.scienta.2006.12.014
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发表时间：
2007-03-26
影响因子：
4.3
通讯作者：
Kakani, V. G.
中科院分区：
文献类型：
--
作者：
Reddy, K. Raja;Kakani, V. G.
成功的坐果取决于几个生殖过程，包括花粉萌发和管生长过程。以辣椒属（Capsicum）品种为材料，采用累积温度反应指数（CTRI）的概念，研究了温度对辣椒花粉萌发特性的影响，并分析了辣椒种/基因型差异。从来自五个辣椒属物种的七个基因型的植物收集花粉，适应世界各地并在大盆中在户外生长。将花粉置于15至50摄氏度的体外温度下，每隔5摄氏度。花粉萌发和管长度记录所有物种在各自的治疗24小时的孵育后。种/基因型差异，显着的花粉离体萌发率和花粉管长度的平均值分别为78%和734 μ m。平均基数温度（T-min，T-opt，和T-max）平均超过基因型，分别为15.2，30.7，和41.8摄氏度花粉萌发和12.2，31.2，和40.4摄氏度的花粉管生长。每个物种/基因型的CTRI计算为8个相对个体胁迫响应值的总和：如最大花粉萌发，最大花粉管长度;花粉萌发的T-min，T-opt和T-max温度，以及花粉管长度，鉴定物种对高温的耐受性。辣椒来自墨西哥的墨西哥塞拉诺（MexSerrano）被鉴定为耐药，C.查科恩斯1312和C. spp. CV. Cobanero分别为中间体和C.紫藤中国早春巨人C. annum cv.来自韩国的Long绿色、C. spp. CV. NM 89 C130和C.毛叶90002从危地马拉敏感的高温。耐受性物种/基因型可用于育种计划中以开发可耐受当前气候和特别是未来更温暖气候中的高温条件的新基因型。(c)2006 Elsevier B. V.保留所有权利。
Successful fruit set depends on several reproductive processes including pollen germination and tube growth processes. An experiment was conducted to determine the effects of temperature on pollen germination characteristics and to identify species/genotypic differences in Capsicum using the cumulative temperature response index (CTRI) concept. Pollen was collected from plants of seven genotypes from five Capsicum species, adapted to various parts of the world and grown outdoors in large pots. The pollen was subjected to in vitro temperatures ranging from 15 to 50 degrees C at 5 degrees C intervals. Pollen germination and tube lengths were recorded for all species after 24 h of incubation at the respective treatments. Species/ genotypes differed, significantly for in vitro pollen germination percentage and pollen tube length with mean values of 78% and 734 mu m, respectively. The mean cardinal temperatures (T-min, T-opt, and T-max) averaged over genotypes, were 15.2, 30.7, and 41.8 degrees C for pollen germination and 12.2, 31.2, and 40.4 degrees C for pollen tube growth. The CTRI of each species/genotype calculated as the sum of eight relative individual stress response values:, such as maximum pollen germination, maximum pollen tube length; T-min, T-opt, and T-max temperatures of pollen germination, and pollen tube lengths, identified species tolerance to high temperatures. Capsicum annum cv. Mex Serrano from Mexico was identified as tolerant, C. chacoense cv. 1312 and C. spp. cv. Cobanero from Argentina and Guatemala, respectively as intermediate and C. frutescens cv. Early Spring Giant from China, C. annum cv. Long Green from South Korea, C. spp. cv. NM89C130 and C. pubescens cv. 90002 from Guatemala as sensitive to high temperatures. The tolerant species/genotypes can be used in breeding programs to develop new genotypes that can withstand high temperature conditions both in the present climate and particularly in a future warmer climate. (c) 2006 Elsevier B.V. All rights reserved.