Revealing Interfacial Lipid Hydrolysis Catalyzed by Phospholipase A(1) at Molecular Level via Sum Frequency Generation Vibrational Spectroscopy and Fluorescence Microscopy
Revealing Interfacial Lipid Hydrolysis Catalyzed by Phospholipase A(1) at Molecular Level via Sum Frequency Generation Vibrational Spectroscopy and Fluorescence Microscopy
复制标题
通过和频产生振动光谱和荧光显微镜揭示分子水平上磷脂酶 A(1) 催化的界面脂质水解
DOI：
10.1021/acs.langmuir.9b02284
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发表时间：
2019
期刊：
影响因子：
3.9
通讯作者：
Lu Xiaolin
中科院分区：
文献类型：
--
作者：
Wang Feng;Li Xu;Zhang Furong;Liu Xiaoyang;Hu Pengcheng;Beke Somfai Tamas;Lu Xiaolin
利用和频振动光谱和荧光显微镜研究了磷脂酶A1（PLA1）催化磷脂的界面水解。使用单层和双层设置来确认水解机制。在水解过程中，水解产物之一溶血磷脂从界面脱附到溶液中，而另一产物脂肪酸则自组织并与PLA1在界面处聚集形成PLA1诱导区，这些区域可作为蛋白质的非特异性结合域，从而导致人类血管疾病。该实验研究为揭示与脂质代谢相关的界面生化过程提供了必要的信息，脂质是细胞的基本组成部分之一。
The interfacial hydrolysis of phospholipids catalyzed by phospholipase A1(PLA1) was studied via sum frequency generation (SFG) vibrational spectroscopy and fluorescence microscopy. Both monolayer and bilayer setups were used to confirm the hydrolysis mechanism. During the hydrolysis, lysophospholipids, one of the hydrolysis products, were desorbed from the interface into the solution, while the other products, fatty acids, self-organized and accumulated with PLA1at the interface to form the PLA1-induced regions, which can serve as nonspecific binding domains for proteins and thus lead to human vascular diseases. This experimental study provides the essential information on revealing the interfacial biochemical process related to the metabolism of the lipids, which is one of the basic building blocks for cells.