Correlation between 31P NMR phosphomonoester and biochemically determined phosphorylethanolamine and phosphatidylethanolamine during development of the rat brain.
Correlation between 31P NMR phosphomonoester and biochemically determined phosphorylethanolamine and phosphatidylethanolamine during development of the rat brain.
复制标题
大鼠大脑发育过程中 31P NMR 磷酸单酯与生化测定的磷酸乙醇胺和磷脂酰乙醇胺之间的相关性。
DOI：
10.1159/000111971
复制
发表时间：
1988
影响因子：
2.9
通讯作者：
N. Herschkowitz
中科院分区：
文献类型：
--
作者：
R. Burri;F. Lazeyras;W. Aue;P. Straehl;P. Bigler;U. Althaus;N. Herschkowitz
通过体内和体外31 P核磁共振（NMR）光谱法以及经典生化方法测量了发育中大鼠大脑中的磷酸单酯。体外NMR显示磷酸单酯峰的主要成分是磷酸乙醇胺。在体内NMR测量磷酸单酯下降，在发展过程中以相同的速率作为生化估计磷酸乙醇胺。磷酸乙醇胺，膜脂质磷脂酰乙醇胺的前体，在发展过程中减少平行的脂质磷脂酰乙醇胺，这是生化测定的增加。这些研究表明，31P NMR可用于监测体内大脑发育。
Phosphomonoesters were measured in the developing rat brain by in vivo and in vitro 31P nuclear magnetic resonance (NMR) spectroscopy and by classical biochemical methods. In vitro NMR showed that the main component of the phosphomonoester peak is phosphorylethanolamine. Phosphomonoesters measured by in vivo NMR decreased during development at the same rate as the biochemically estimated phosphorylethanolamine. Phosphorylethanolamine, a precursor of the membrane lipid phosphatidylethanolamine, decreased during development parallel to an increase of the lipid phosphatidylethanolamine, which was measured biochemically. These studies show that 31P NMR can be used to monitor brain development in vivo.