The Bacillus subtilis menCD promoter is responsive to extracellular pH.
The Bacillus subtilis menCD promoter is responsive to extracellular pH.
复制标题
枯草芽孢杆菌 menCD 启动子对细胞外 pH 值有反应。
DOI：
10.1007/bf00249005
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发表时间：
1990
影响因子：
2.8
通讯作者：
Taber,HW
中科院分区：
文献类型：
--
作者：
Hill,KF;Mueller,JP;Taber,HW
利用amenCD′-lacZ翻译基因融合技术，研究了枯草芽孢杆菌萘醌生物合成基因启动子（enCD启动子）在生长和产孢过程中的激活动力学。在含有低葡萄糖浓度的非缓冲复合培养基中，指数生长结束后不久，即可观察到瞬时最大激活。这些激活动力学与短暂酸化的条件下，允许TCA循环功能在指数期后的介质，而突变，阻止TCA循环功能（citmutants）与持续酸化和启动子激活在此期间。Incit+菌株中，将培养基缓冲至pH 5.7引起持续的最大活化，而缓冲至pH 7.2防止活化的增强。ThemenCD启动子似乎对细胞外酸性pH有反应。
Activation kinetics of aBacillus subtilismenaquinone biosynthetic gene promoter (themenCDpromoter) were measured during growth and sporulation, with the aid of amenCD′-lacZtranslational gene fusion. Transient maximal activation was seen shortly after the end of exponential growth in unbuffered complex medium containing a low glucose concentration. These activation kinetics were correlated with transient acidification of the medium under conditions permitting TCA cycle function during the post-exponential period, while mutations that blocked TCA cycle function (citmutants) were associated with sustained acidification and promoter activation during this period. Incit+strains, buffering of the medium to pH 5.7 caused sustained maximal activation, while buffering to pH 7.2 prevented enhancement of activation. ThemenCDpromoter appears to be responsive to extracellular acidic pH.