Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide
Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide
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嘌呤环酰胺的基因组挖掘和酶法全生物合成
DOI：
10.1021/acs.orglett.9b02461
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发表时间：
2019-09-06
期刊：
影响因子：
5.2
通讯作者：
Ge, Hui Ming
中科院分区：
文献类型：
--
作者：
Shi, Jing;Xu, Xiang;Ge, Hui Ming
通过基因组挖掘，在恰塔努加链霉菌（Streptomyces chrestomyceticus）NA4264中鉴定出一个含环二肽合酶的基因簇（pcm）。该隐性pcm基因簇在白色链霉菌（Streptomyces albus）中的异源表达产生了一种新的高度修饰的环二肽，名为嘌呤环酰胺（1）。生物信息学分析和基因敲除实验揭示了其生物合成途径。化合物1通过一锅法酶促反应实现合成。
A cyclodipeptide synthase-containing gene cluster (pcm) was identified in Streptomyces chrestomyceticus NA4264 through genome mining. Heterologous expression of the cryptic pcm gene cluster in Streptomyces albus led to the production of a new highly modified cyclodipeptide named purincyclamide (1). Bioinformatic analysis and gene knockout experiments revealed the biosynthetic pathway. The production of 1 was achieved through a one-pot enzymatic reaction.