Metabolic flux analysis of the phenylpropanoid pathway in elicitor-treated potato tuber tissue
Metabolic flux analysis of the phenylpropanoid pathway in elicitor-treated potato tuber tissue
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DOI：
10.1093/pcp/pci042
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发表时间：
2005-03-01
影响因子：
4.9
通讯作者：
Miyagawa, H
中科院分区：
文献类型：
--
作者：
Matsuda, F;Morino, K;Miyagawa, H
通过监测7种化合物含量的时间变化，定量分析了β-1，3-寡糖激发子对马铃薯块茎中苯丙烷代谢的影响。激发子处理增加了章鱼胺和酪胺酰胺(N-对香豆酰酪胺、N-阿魏酰酪胺、N-对香豆酰酪胺和N-阿魏酰酪胺)的池大小，而减少了绿原酸和腐胺(咖啡酰腐胺和阿魏酰腐胺)的池大小。利用稳定同位素标记和LC-MS检测对代谢通量的分析清楚地表明，这些化合物的池大小的变化与激发子处理后它们的生物合成通量(J(In))的变化相关。在章鱼胺和酪氨酰胺的情况下，J(In)的增加伴随着转化通量(J(Out))的增加，这表明这些化合物在激发子处理的块茎组织中迅速周转。通量分析的结果表明，激发子处理后，章鱼胺和酪氨酰胺生物合成的实际激活程度大于从马铃薯组织中它们的水平变化估计的激活程度。这些发现表明，这些酰胺类化合物及其代谢衍生物在马铃薯中与防御相关的苯丙醇代谢中起着重要作用。
The effects of beta-1,3-oligosaccharide elicitor on the metabolism of phenylpropanoids in potato tuber were analyzed quantitatively, by monitoring the time-dependent changes in the levels of seven compounds. The elicitor treatment caused an increase in the pool size of octopamine and tyramine amides (N-p-coumaroyloctopamine, N-feruloyloctopamine, N-p-coumaroyltyramine and N-feruloyltyramine), as well as a decrease in that of chlorogenic acid and putrescine amides (caffeoylputrescine and feruloylputrescine). An analysis of metabolic flux using stable isotope labeling and liquid chromatography-spectrometry (LC-MS) detection clearly demonstrated that the changes in the pool size of these compounds were correlated with the changes in their flux for biosynthesis (J(in)) upon elicitor treatment. The increase in J(in) in the cases of octopamine and tyramine amides was accompanied by an increase in flux for the transformation (J(out)), indicating a rapid turnover of these compounds in the elicitor-treated tuber tissue. The result of the flux analysis indicated that the actual activation of the biosynthesis of octopamine and tyramine amides after the elicitor treatment was greater than that estimated from the changes in their levels in the potato tissue. These findings suggest that these amide compounds and their metabolic derivatives play an important role in the defense-related metabolism of phenylpropanoids in potato.