Evaluating uncertainty in the calculation of non-exchangeable hydrogen fractions within organic materials
Evaluating uncertainty in the calculation of non-exchangeable hydrogen fractions within organic materials
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DOI：
10.1002/rcm.4000
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发表时间：
2009-05-01
影响因子：
2
通讯作者：
Ehleringer, James R.
中科院分区：
文献类型：
--
作者：
Chesson, Lesley A.;Podlesak, David W.;Ehleringer, James R.
我们计算了蛋白质物质中可交换氢原子的比例，这些蛋白质物质通常用于分析与动物原产地有关的稳定同位素组成。其中包括几种类型的角蛋白和角蛋白，以及肌肉组织。我们发现，在生物相关温度（25摄氏度）下，角蛋白中可用于交换的H原子的比例在一系列地面有机材料中平均为9%，但在剪下的头发中高达17%；肌肉组织中有12%的可交换氢原子。在大多数分析条件下，由于物理样品处理而导致的可交换分数的差异对含角蛋白组织中不可交换H原子的计算δ H-2值的影响很小(
We calculated the fraction of exchangeable hydrogen atoms in proteinaceous materials commonly analyzed for stable isotopic composition related to the region-of-origin of an animal. These included several types of alpha- and beta-keratin, and muscle tissue. We find that the fraction of H atoms in keratin available for exchange at a biologically relevant temperature (25 degrees C) averaged 9% across a range of ground organic materials, but was as high as similar to 17% in cut hair; muscle tissue has similar to 12% exchangeable H atoms. Under most analysis conditions, the difference in exchangeable fractions due to physical sample processing has a minimal effect on the calculated delta H-2 values of the non-exchangeable H atoms within a keratin-containing tissue (