Time profile of putrescine, cadaverine, indole and skatole in human saliva
Time profile of putrescine, cadaverine, indole and skatole in human saliva
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DOI：
10.1016/s0003-9969(03)00015-3
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发表时间：
2003-04-01
影响因子：
3
通讯作者：
White, C
中科院分区：
文献类型：
--
作者：
Cooke, M;Leeves, N;White, C
腐胺，尸胺，吲哚和粪臭素的浓度进行了测定，在清醒时，在一天中的时间点的健康志愿者的唾液中。腐胺被认为是最丰富的胺的研究，其次是尸胺，然后吲哚。粪臭素在任何时间点的唾液样本中均未检出。这些胺在刚醒来时浓度最高（平均浓度（μ g/ml）：腐胺33.0 +/- 19.0，尸胺17.6 +/- 16.7和吲哚0.4 +/- 0.4），在吃早餐和刷牙后迅速下降（平均浓度（μ g/ml）：腐胺7.0 +/- 6.4，尸胺3.1 +/- 4.7和吲哚0.04 +/- 0.09）。腐胺和尸胺的浓度在白天增加，除了由于咀嚼引起的唾液流量增加和机械清洁引起的午餐后降低。建立了一种基于高效液相色谱-荧光检测的唾液胺定量分析方法。氟化钠已被证明是在室温下唾液中胺形成的有效抑制剂，允许样品被收集和保存而不需要冷藏。(C)2003爱思唯尔科技有限公司版权所有。
The concentrations of putrescine, cadaverine, indole and skatole were determined in the saliva of healthy human volunteers upon waking and at time points during the day. Putrescine was found to be the most abundant of the amines studied, followed by cadaverine then indole. Skatole could not be detected in the saliva samples at any time point. The amines were found in the highest concentrations immediately upon waking (mean concentrations (mug/ml): putrescine 33.0 +/- 19.0, cadaverine 17.6 +/- 16.7 and indole 0.4 +/- 0.4) with a rapid decrease following consumption of breakfast and brushing their teeth (mean concentrations (mug/ml): putrescine 7.0 +/- 6.4, cadaverine 3.1 +/- 4.7 and indole 0.04 +/- 0.09). Putrescine and cadaverine then increased in concentration during the day apart from a decrease post-lunch caused by increased salivary flow and mechanical cleaning due to mastication. An analytical method based on high performance liquid chromatography and fluorescence detection has been developed to quantify amines in human saliva. Sodium fluoride has been shown to be an effective inhibitor of amine formation in saliva at room temperature allowing samples to be collected and kept without requiring cold storage. (C) 2003 Elsevier Science Ltd. All rights reserved.