Identification and characterization of an apoptosis-stimulating protein of p53 (ASPP) gene from Branchiostoma belcheri: Insights into evolution of ASPP gene family
Identification and characterization of an apoptosis-stimulating protein of p53 (ASPP) gene from Branchiostoma belcheri: Insights into evolution of ASPP gene family
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DOI：
10.1016/j.fsi.2015.12.040
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ma Fei
中科院分区：
文献类型：
--
作者：
Song Xiaojun;Du Juan;Zhu Wei;Jin Ping;Ma Fei
The ASPP (apoptosis-stimulating protein of p53) protein family plays very key roles in apoptosis regulation, in both p53-dependent and p53-independent pathways. However, the ASPP homologous gene has.not been identified in amphioxus to date. Here, we identified and characterized an ASPP gene from.Branchiostoma belcheri (designed as AmphiASPP) and extensively studied its evolution and roles involved.in innate immunity. The results showed that the amphioxus genome has an ASPP homolog gene with an.ORF of 3285 bp, encoding 1094 amino acids which contains ANK repeats and SK3 domain. The evolutionary analyses indicated that the members of ASPP protein family might be present in a common.ancestor of Nematostella vectensis and underwent positive selective in the evolutionary history. In.addition, the amphioxus ASPP gene was ubiquitously and differentially expressed in five investigated.tissues, and the amphioxus ASPP gene was involved in the innate immune response of LPS and LTA.stimulation. Finally, bioinformatic analyses displayed that amphioxus ASPP protein could interact with.REL protein by conserved binding sites compared with human ASPP2 protein, which seemed to further.suggest that the amphioxus ASPP protein involve in innate immunity through NF-кB signaling pathway..Taken together, our findings provided an insight into the evolution and innate immunity function of the.ASPP family.
