A NEW ACID-TOLERANT NITROGEN-FIXING BACTERIUM ASSOCIATED WITH SUGARCANE
A NEW ACID-TOLERANT NITROGEN-FIXING BACTERIUM ASSOCIATED WITH SUGARCANE
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DOI：
10.1007/bf02370096
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发表时间：
1988-05-01
期刊：
影响因子：
4.9
通讯作者：
DOBEREINER, J
中科院分区：
文献类型：
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作者：
CAVALCANTE, VA;DOBEREINER, J
在对甘蔗中可能负责固氮的细菌进行调查时，在巴西的四个甘蔗种植区收集了根和茎样本。在所有地区的大部分洗根和洗茎样品中分离到一种新的微生物固氮菌。分离程序基于半固体稀释甘蔗汁培养基，然后复制到无n 10%糖培养基，乙酸酸化至pH 4.5。这种新细菌是一种好氧棒，通过1到3根侧鞭毛运动，在空气下的半固体培养基中固定n2，但在液体培养基中不固定n2，除非加入起始剂量的N。它没有硝酸盐，在10mMNO3−存在下进行还原酶和n2固定。高蔗糖浓度（10%）时生长最佳。蔗糖含量在30%时生长，而在35%时生长不了。最终pH值低于3.0时形成酸。生长和固氮在这种酸度下进行。乙醇用于生长，并被“过度氧化”（氧化为co2和H2O）。乙酸和乳酸也被氧化成二氧化碳和水。葡萄糖产生的酸随着CaCO3的沉淀而消耗。深褐色菌落落在含10%糖的马铃薯琼脂上，深橙色菌落落在含溴百里香酚蓝的N差琼脂上（每1粒20 mg酵母提取物）。鉴于其独特的特性，不允许在Frateuria， Gluconobacter， Acetobacter或任何已知的固氮细菌中进行鉴定，提出了一个新的属和种，并命名为saccharobacter nitrocaptans。
During surveys of bacteria possibly responsible for N2fixation in sugarcane, root and stem samples were collected in four sugarcane-growing regions in Brazil. A new microaerobic N2-fixing bacterium was isolated from most samples of washed roots and stems from all regions. Isolation procedures were based on semisolid diluted sugarcane juice medium followed by replication to N-free 10% sugar medium acidified with acetic acid to pH 4.5. The new bacterium is an aerobic rod, motile by 1 to 3 lateral flagella, fixes N2in semisolid media under air but not in liquid media except when a starter dose of N is added. It has no nitrate, reductase and N2fixation proceeds in the presence of 10mMNO3−. Best growth occurs with high sucrose concentrations (10%). Growth occurs up to 30% sucrose but not at 35%. Acid is formed reaching a final pH of below 3.0. Growth and N2fixation proceed at this acidity. Ethanol is used for growth and is “overoxidised” (oxidized to CO2and H2O). Acetic and lactic acids are also oxidized to CO2and H2O. Acids produced from glucose are consumed with precipitation of CaCO3. Dark brown colonies are formed on potato agar with 10% sugar and dark orange colonies on N poor agar (20 mg yeast extract per 1) containing bromothymol blue. In view of the distinct characteristics which do not allow identification within either Frateuria, Gluconobacter, Acetobacter or any known N2-fixing bacterium a new genus and species are proposed and namedSaccharobacter nitrocaptans.