Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines
Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines
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DOI：
10.1006/viro.2002.1615
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发表时间：
2002-11-10
期刊：
影响因子：
3.7
通讯作者：
Aasheim, HC
中科院分区：
文献类型：
--
作者：
Patzke, S;Lindeskog, M;Aasheim, HC
我们已经在人类前B细胞白血病细胞系REH中鉴定了一个人内源性逆转录病毒(HERV)Gag转录本。该转录本被发现是位于染色体6q13上的结构完整的Herv元件的剪接产物。它的引物结合位点与HERV-F家族常见的苯丙氨酸(F)tRNA互补，但整个基因组序列与HERV-H家族密切相关。因此，逆转录病毒序列被命名为HERV-H/F。HERV元件在造血癌细胞系中表现出独特的mRNA表达模式，在一些B淋巴系和髓系白血病来源的白血病细胞系中表达。在正常人体组织中没有观察到表达，这表明了一种癌症特异性的表达模式。在表达和不表达HERV-H/F的细胞系中检测5‘长末端重复序列(LTR)启动子活性。LTR介导的报告基因表达的细胞特异性与内源病毒的表达没有明确的相关性，表明该基因的转录调控不是单独依赖于转录因子的细胞特异性活性。(C)2002年埃尔塞维尔科学公司(美国)。
We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA).