CATION-EXCHANGE CHROMATOGRAPHY OF PEPTIDES ON POLY(2-SULFOETHYL ASPARTAMIDE)-SILICA
CATION-EXCHANGE CHROMATOGRAPHY OF PEPTIDES ON POLY(2-SULFOETHYL ASPARTAMIDE)-SILICA
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DOI：
10.1016/s0021-9673(00)94785-x
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发表时间：
1988-06-29
期刊：
影响因子：
--
通讯作者：
ANDREWS, PC
中科院分区：
文献类型：
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作者：
ALPERT, AJ;ANDREWS, PC
开发了一种强阳离子交换材料聚(2-磺乙基天冬氨酰胺)-硅胶(聚磺乙基天冬氨酰胺)，用于多肽的纯化和高效液相色谱分析。即使氨基末端是唯一的碱性基团，所考察的所有多肽也能在pH 3下保留。多肽按碱性残基数量递增的顺序随盐梯度洗脱。这种新的柱材料表现出温和的混合模式效应，允许在给定的pH下拆分具有相同电荷的多肽。选择性可以通过在流动相中加入有机溶剂来控制，这增加了一些多肽的保留率，降低了另一些多肽的保留率。在任何给定情况下的保留可能反映了空间因素和非静电相互作用的组合。选择性是对反相色谱(RPC)材料的补充。采用Poly-SUFLOEHYL-ASPARAY和RPC连续柱分离纯化组织粗提物中的多肽，获得了较好的纯化效果。新型阳离子交换剂作为RPC在一般多肽层析中的补充具有很好的应用前景。
A strong cation-exchange material, poly(2-sulfoethyl aspartamide)-silica (PolySULFOETHYL Aspartamide) was developed for purification and analysis of peptides by high-performance liquid chromatography. All peptides examined were retained at pH 3, even when the amino terminus was the only basic group. Peptides were eluted in order of increasing number of basic residues with a salt gradient. Capacity was high, as was selectivity and column efficiency.This new column material displays modest mixed-mode effects, allowing the resolution of peptides having identical charges at a given pH. The selectivity can be manipulated by the addition of organic solvent to the mobile phases; this increases the retention of some peptides and decreases the retention of others. The retention in any given case may reflect a combination of steric factors and non-electrostatic interactions.Selectivity was complementary to that of reversed-phase chromatography (RPC) materials. Excellent purifications were obtained by sequential use of Poly-SUFLOETHYL Aspartamide and RPC columns for purification of peptides from crude tissue extracts. The new cation exchanger is quite promising as a supplement to RPC for general peptide chromatography.